THE SIMIAN VIRUS-40 SMALL-T INTRON, PRESENT IN MANY COMMON EXPRESSION VECTORS, LEADS TO ABERRANT SPLICING

被引：95

作者：

HUANG, MTF ^{[1
]}

GORMAN, CM ^{[1
]}

机构：

[1] GENENTECH INC, DEPT CELL GENET, 460 POINT SAN BRUNO BLVD, San Francisco, CA 94080 USA

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 04期

关键词：

D O I：

10.1128/MCB.10.4.1805

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polymerase chain reaction analysis was used to identify aberrant splicing of the simian virus 40 small-t intron present in pRSV cat. We examined factors governing the selection and relative use of aberrant 5' splice sites derived from the chloramphenicol acetyltransferase-coding region. Our results indicated that transcripts from virtually any cDNA positioned upstream of the small-t intron could contain alternative 5' splice sites and therefore be subject to deletions within the protein-coding region.

引用

页码：1805 / 1810

页数：6

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