CHARACTERIZATION OF GLUCOSE-6-PHOSPHATE-DEHYDROGENASE AND 6-PHOSPHOGLUCONIC ACID DEHYDROGENASE FROM HAZEL COTYLEDONS

被引：16

作者：

GOSLING, PG

ROSS, JD

机构：

[1] Dept. of Botany, University of Reading, Reading, Berkshire

来源：

PHYTOCHEMISTRY | 1979年 / 18卷 / 09期

关键词：

6-phosphogluconic acid dehydrogenase; Corylaceae; Corylus avellana; glucose-6-phosphate dehydrogenase; inherent NADP reduction; pentose phosphate metabolism;

D O I：

10.1016/S0031-9422(00)98472-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

NADP reduction was shown to occur in a crude cytosolic extract from the cotyledonary material of hazel seed prior to the addition of erogenous dehydrogenase substrate. This activity interfered with the assay of glucose-6-phosphate dehydrogenase and 6-phosphogluconic acid dehydrogenase activities. The inherent NADP reduction was removed by ammonium sulphate fractionation. Subsequent de-salting of the resulting partially-purified fraction permitted assay of G6PDH and 6PGDH. Both enzymes were shown to be NADP specific. Typical Michaelis-Menten kinetics were shown for each enzyme, towards NADP and their respective substrate. © 1979.

引用

页码：1441 / 1445

页数：5

共 23 条

[1] GLUCOSE DEHYDROGENASE ACTIVITY OF YEAST GLUCOSE 6-PHOSPHATE DEHYDROGENASE .2. KINETIC STUDIES OF MODE OF ACTIVATION BY BICARBONATE PHOSPHATE PHOSPHATE AND SULFATE [J].