NEW MODEL OF TERTIARY STRUCTURE OF PLANT 5S RIBOSOMAL-RNA IS CONFIRMED BY DIGESTIONS WITH ALPHA-SARCIN

被引：8

作者：

BARCISZEWSKA, MZ

LORENZ, S

JOACHIMIAK, A

ULBRICH, N

ERDMANN, VA

BARCISZEWSKI, J

机构：

[1] FREE UNIV BERLIN,INST BIOCHEM,THIELALLEE 63,W-1000 BERLIN 33,GERMANY

[2] POLISH ACAD SCI,INST BIOORGAN CHEM,PL-61704 POZNAN,POLAND

来源：

FEBS LETTERS | 1990年 / 269卷 / 01期

关键词：

Plant 5S rRNA; Tertiary structure model; α-Sarcin;

D O I：

10.1016/0014-5793(90)81124-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cytotoxin α-sarcin was employed to test the model of secondary and tertiary structures of plant 5S rRNAs, which we recently proposed [(1990) Int. J. Biol. Macromol. (in press)]. α-Sarcin is a novel ribonuclease that hydrolyzes phosphodiester bonds adjacent to purines in nucleic acids [2-4]. The digestion pattern obtained for lupin and wheat germ 5S rRNAs strongly suggests the existence of tertiary interactions between residues C34, C35, C36, A37 and G85, G86, 087, U88 as previously proposed. The results on the secondary structure of plant 5S rRNA are in line with a previously proposed model [5]. © 1990.

引用

页码：83 / 85

页数：3

共 24 条

[1]

ACKERMAN EJ, 1988, J BIOL CHEM, V263, P17076

[2] THE NUCLEOTIDE-SEQUENCE OF METHIONINE ELONGATOR TRANSFER-RNA FROM WHEAT-GERM [J].

BARCISZEWSKA, M ;

DIRHEIMER, G ;

KEITH, G .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1983, 114 (03) :1161-1168

[3] THE PRIMARY STRUCTURE OF MAIZE AND TOBACCO 5-S RIBOSOMAL-RNA [J].

BARCISZEWSKA, MZ ;

MASHKOVA, TD ;

KISSELEV, LL ;

BARCISZEWSKI, J .

FEBS LETTERS, 1985, 192 (02) :289-293

[4]

DIGWEED M, 1982, EUR J BIOCHEM, V127, P531

[5] IMPROVED PROCEDURE FOR THE ISOLATION OF A DOUBLE-STRAND-SPECIFIC RIBONUCLEASE AND ITS APPLICATION TO STRUCTURAL-ANALYSIS OF VARIOUS 5S RIBOSOMAL-RNAS AND TRANSFER-RNAS [J].

DIGWEED, M ;

PIELER, T ;

KLUWE, D ;

SCHUSTER, L ;

WALKER, R ;

ERDMANN, VA .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 154 (01) :31-39

[6]

DIGWEED M, 1986, ADV METHODS PROTEIN, P364

[7] MAPPING ADENINES, GUANINES, AND PYRIMIDINES IN RNA [J].

DONISKELLER, H ;

MAXAM, AM ;

GILBERT, W .

NUCLEIC ACIDS RESEARCH, 1977, 4 (08) :2527-2537

[8] PROBING THE STRUCTURE OF RNAS IN SOLUTION [J].

EHRESMANN, C ;

BAUDIN, F ;

MOUGEL, M ;

ROMBY, P ;

EBEL, JP ;

EHRESMANN, B .

NUCLEIC ACIDS RESEARCH, 1987, 15 (22) :9109-9128

[9]

ENDO Y, 1982, J BIOL CHEM, V257, P9054

[10]

ENDO Y, 1988, J BIOL CHEM, V263, P7917

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