CHARACTERIZATION OF A MONOCLONAL-ANTIBODY AGAINST NATIVE HUMAN TYPE-I COLLAGEN

被引：43

作者：

WERKMEISTER, JA

RAMSHAW, JAM

ELLENDER, G

机构：

[1] CSIRO,DIV BIOTECHNOL,343 ROYAL PARADE,PARKVILLE,VIC 3052,AUSTRALIA

[2] UNIV MELBOURNE,SCH DENT SCI,PARKVILLE,VIC 3052,AUSTRALIA

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1990年 / 187卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1990.tb15323.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A monoclonal antibody against a pepsin‐soluble mammalian type I collagen has been produced. This antibody, subclass IgG1,K, was specific for type I collagen and did not cross‐react with a range of other collagen types or connective tissue proteins. The epitope recognized by the antibody was dependent upon an intact triple‐helical structure for the collagen, and was shown by rotary shadowing and by immunoblotting of collagenase‐derived fragments to be near the C‐terminal of the pepsin‐soluble collagen. Although the antibody had a low affinity, with Kd= 4×10−7 M, it could be used for immunohistology of tissue sections and for studies of collagen produced by cells in culture. The antibody, which was raised against human collagen, also recognized type I collagens from certain other species, including calf, pig, sheep, goat and dog. Copyright © 1990, Wiley Blackwell. All rights reserved

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页码：439 / 443

页数：5

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