CYCLIC-AMP RELAXES SWINE ARTERIAL SMOOTH-MUSCLE PREDOMINANTLY BY DECREASING CELL CA2+ CONCENTRATION

被引:62
作者
MCDANIEL, NL
REMBOLD, CM
RICHARD, HM
MURPHY, RA
机构
[1] UNIV VIRGINIA,HLTH SCI CTR,DEPT PEDIAT,DIV CARDIOL,BOX 146,CHARLOTTESVILLE,VA 22908
[2] UNIV VIRGINIA,HLTH SCI CTR,DEPT INTERNAL MED & PHYSIOL,DIV CARDIOL,CHARLOTTESVILLE,VA 22908
来源
JOURNAL OF PHYSIOLOGY-LONDON | 1991年 / 439卷
关键词
D O I
10.1113/jphysiol.1991.sp018661
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1. Our objective was to evaluate the mechanism of cyclic AMP-dependent arterial smooth muscle relaxation. Cyclic AMP-dependent relaxation has been proposed to result from either (a) a decrease in intracellular [Ca2+] or (b) a decreasse in [Ca2+] sensitivity of myosin light chain kinase by protein kinase A-dependent phosphorylation of myosin kinase. 2. We evaluated these proposed mechanisms by examining forskolin-induced changes in aequorin-estimated myoplasmic [Ca2+], [cyclic AMP], myosin phosphorylation and stress generation in agonist-stimulated or KCl-depolarized swine common carotid media tissues. 3. Forskolin, an activator of adenylyl cyclase, increased [cyclic AMP] and reduced [Ca2+], myosin phosphorylation and stress in tissues pre-contracted with phenylephrine or histamine. This relaxation was not associated with an alteration of the [Ca2+] sensitivity of phosphorylation, nor the dependence of stress on phosphorylation. 4. Forskolin pre-treatment attenuated, but did not abolish, agonist-induced increases in [Ca2+] and stress. 5. These results suggest that cyclic AMP-induced relaxation of the agonist-stimulated swine carotid media is primarily caused by cyclic AMP-mediated decreases in myoplasmic [Ca2+].
引用
收藏
页码:147 / 160
页数:14
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