AN INTER-LABORATORY STUDY OF STARCH DEGRADING ENZYME ASSAYS

被引：3

作者：

HENRY, RJ ^{[1
]}

BUTLER, D ^{[1
]}

机构：

[1] QUEENSLAND DEPT PRIMARY IND,TOOWOOMBA,QLD 4350,AUSTRALIA

来源：

JOURNAL OF THE INSTITUTE OF BREWING | 1992年 / 98卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

ALPHA-AMYLASE; BETA-AMYLASE; LIMIT DEXTRINASE; COLLABORATIVE STUDY;

D O I：

10.1002/j.2050-0416.1992.tb01087.x

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The reliability of specific colorimetric assays for the main starch degrading enzymes, alpha-amylase, beta-amylase and limit dextrinase was investigated by conducting an inter-laboratory collaborative study. Three malt samples were assayed in duplicate in 14 laboratories. The repeatability (r) within laboratories was good for alpha-amylase and beta-amylase assays. Between-laboratory reproducibility (R) was acceptable (CV(R) 9.2-14%). Errors for limit dextrinase were higher. Repeatability was good at high enzyme concentrations (CV(r) = 2%) but increased at low enzyme concentrations. Reproducibility was relatively poor (CV(R)-27-33%).

引用

页码：25 / 26

页数：2