TRANSIENT INCREASE IN VIMENTIN PHOSPHORYLATION AND VIMENTIN-HSC70 ASSOCIATION IN 9L RAT-BRAIN TUMOR-CELLS EXPERIENCING HEAT-SHOCK

被引:27
作者
CHENG, TJ [1 ]
LAI, YK [1 ]
机构
[1] NATL TSING HUA UNIV,INST LIFE SCI,HSINCHU 30043,TAIWAN
关键词
HEAT-SHOCK GENES; HSC70; VIMENTIN; PROTEIN PHOSPHORYLATION; INTERMEDIATE FILAMENTS;
D O I
10.1002/jcb.240540111
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Characteristic changes in vimentin were studied in 9L rat brain tumor cells treated at 45 degrees C. During heat-shock treatment, vimentin molecules were rapidly phosphorylated and reorganized from a filamentous form into a perinuclear higher-order structure that was less extractable by nonionic detergent. These effects were found to be highly transient, peaked at 30 min after the onset of heat-shock treatment, and subsided thereafter. Simultaneously, the solubility of the constitutively expressed heat-shock protein70 (HSC70) was also temporarily decreased and the kinetics was identical to that of vimentin. The results indicated that HSC70 and vimentin were co-insolubilized during the heat-shock treatment. We propose that the reorganization of the intermediate filaments resulted from enhanced phosphorylation of vimentin leads to the concurrent association of HSC70 to the intermediate filaments. This process may play an essential role in regulating heat-shock genes. (C) 1994 Wiley-Liss, Inc.
引用
收藏
页码:100 / 109
页数:10
相关论文
共 67 条
[1]   THE HUMAN HEAT-SHOCK PROTEIN HSP70 INTERACTS WITH HSF, THE TRANSCRIPTION FACTOR THAT REGULATES HEAT-SHOCK GENE-EXPRESSION [J].
ABRAVAYA, K ;
MYERS, MP ;
MURPHY, SP ;
MORIMOTO, RI .
GENES & DEVELOPMENT, 1992, 6 (07) :1153-1164
[2]  
ANATHAN J, 1986, SCIENCE, V232, P522
[3]   HEAT-SHOCK GENE-REGULATION BY NASCENT POLYPEPTIDES AND DENATURED PROTEINS - HSP70 AS A POTENTIAL AUTOREGULATORY FACTOR [J].
BALER, R ;
WELCH, WJ ;
VOELLMY, R .
JOURNAL OF CELL BIOLOGY, 1992, 117 (06) :1151-1159
[4]   EXAMINING THE FUNCTION AND REGULATION OF HSP-70 IN CELLS SUBJECTED TO METABOLIC STRESS [J].
BECKMANN, RP ;
LOVETT, M ;
WELCH, WJ .
JOURNAL OF CELL BIOLOGY, 1992, 117 (06) :1137-1150
[5]  
Bershadsky AD., 1988, CYTOSKELETON, V1st
[6]   HEAT-SHOCK AND THE HEAT-SHOCK PROTEINS [J].
BURDON, RH .
BIOCHEMICAL JOURNAL, 1986, 240 (02) :313-324
[7]  
CABRAL F, 1979, J BIOL CHEM, V254, P6203
[8]   PHOSPHORYLATION OF KERATIN AND VIMENTIN POLYPEPTIDES IN NORMAL AND TRANSFORMED MITOTIC HUMAN EPITHELIAL AMNION CELLS - BEHAVIOR OF KERATIN AND VIMENTIN FILAMENTS DURING MITOSIS [J].
CELIS, JE ;
LARSEN, PM ;
FEY, SJ ;
CELIS, A .
JOURNAL OF CELL BIOLOGY, 1983, 97 (05) :1429-1434
[9]   UNCOATING ATPASE IS A MEMBER OF THE 70 KILODALTON FAMILY OF STRESS PROTEINS [J].
CHAPPELL, TG ;
WELCH, WJ ;
SCHLOSSMAN, DM ;
PALTER, KB ;
SCHLESINGER, MJ ;
ROTHMAN, JE .
CELL, 1986, 45 (01) :3-13
[10]   PHOSPHORYLATION AND DISASSEMBLY OF INTERMEDIATE FILAMENTS IN MITOTIC CELLS [J].
CHOU, YH ;
ROSEVEAR, E ;
GOLDMAN, RD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (06) :1885-1889