DECREASED MOLECULAR CHAPERONE PROPERTY OF ALPHA-CRYSTALLINS DUE TO POSTTRANSLATIONAL MODIFICATIONS

被引：122

作者：

CHERIAN, M ^{[1
]}

ABRAHAM, EC ^{[1
]}

机构：

[1] MED COLL GEORGIA,DEPT BIOCHEM & MOLEC BIOL,AUGUSTA,GA 30912

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 208卷 / 02期

关键词：

D O I：

10.1006/bbrc.1995.1391

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We studied the effect of oxidation, mixed disulfide formation and glycation of alpha-crystallins on their molecular chaperone property. The ability of alpha-crystallins to protect heat-induced denaturation and aggregation of beta L-crystallin was significantly diminished by these modifications. alpha-Crystallin from senile human lenses also showed significant loss of chaperone-like property. Age-dependent increase in posttranslationally modified alpha-crystallins is the likely cause for this change. (C) 1995 Academic Press. Inc.

引用

页码：675 / 679

页数：5

共 27 条

[1] SITE SELECTIVITY IN THE GLYCATION OF ALPHA-A-CRYSTALLIN AND ALPHA-B-CRYSTALLIN BY GLUCOSE [J].

ABRAHAM, EC ;

CHERIAN, M ;

SMITH, JB .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 201 (03) :1451-1456

[2]

ABRAHAM EC, 1989, MAILLARD REACTION AG, P123

[3] CONFORMATIONAL-CHANGES INDUCED IN LENS ALPHA-CRYSTALLINS AND GAMMA-CRYSTALLINS BY MODIFICATION WITH GLUCOSE-6-PHOSPHATE - IMPLICATIONS FOR CATARACT [J].

BESWICK, HT ;

HARDING, JJ .

BIOCHEMICAL JOURNAL, 1987, 246 (03) :761-769

[4] ALPHA-B SUBUNIT OF LENS-SPECIFIC PROTEIN ALPHA-CRYSTALLIN IS PRESENT IN OTHER OCULAR AND NON-OCULAR TISSUES [J].

BHAT, SP ;

NAGINENI, CN .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 158 (01) :319-325

[5]

BHAT SP, 1991, EUR J BIOCHEM, V102, P775

[6]

BRADFORD MM, 1975, ANAL BIOCHEM, V72, P248

[7] A NEW MIXED DISULFIDE SPECIES IN HUMAN CATARACTOUS AND AGED LENSES [J].

DICKERSON, JE ;

LOU, MF .

BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1157 (02) :141-146

[8] EXPRESSION OF THE MURINE ALPHA-B-CRYSTALLIN GENE IS NOT RESTRICTED TO THE LENS [J].

DUBIN, RA ;

WAWROUSEK, EF ;

PIATIGORSKY, J .

MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (03) :1083-1091

[9] A DIRECT CORRELATION BETWEEN THE LEVELS OF ASCORBIC-ACID AND H2O2 IN AQUEOUS-HUMOR [J].

GIBLIN, FJ ;

MCCREADY, JP ;

KODAMA, T ;

REDDY, VN .

EXPERIMENTAL EYE RESEARCH, 1984, 38 (01) :87-93

[10] THE ABILITY OF LENS ALPHA-CRYSTALLIN TO PROTECT AGAINST HEAT-INDUCED AGGREGATION IS AGE-DEPENDENT [J].

HORWITZ, J ;

EMMONS, T ;

TAKEMOTO, L .

CURRENT EYE RESEARCH, 1992, 11 (08) :817-822

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