ESCHERICHIA-COLI HEAT-LABILE ENTEROTOXIN-B SUBUNITS SUPPLEMENTED WITH A TRACE AMOUNT OF THE HOLOTOXIN AS AN ADJUVANT FOR NASAL INFLUENZA VACCINE

被引:70
作者
TAMURA, S
ASANUMA, H
TOMITA, T
KOMASE, K
KAWAHARA, K
DANBARA, H
HATTORI, N
WATANABE, K
SUZUKI, Y
NAGAMINE, T
AIZAWA, C
OYA, A
KURATA, T
机构
[1] KITASATO INST,DEPT TECHNOL,TOKYO 108,JAPAN
[2] UNIV TOKYO,INST MED SCI,DEPT BACTERIOL,TOKYO,JAPAN
关键词
INFLUENZA VACCINE; ESCHERICHIA-COLI ENTEROTOXIN B SUBUNIT; ADJUVANT;
D O I
10.1016/0264-410X(94)90177-5
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Escherichia coli heat-labile enterotoxin B subunit (LTB) (2 mu g), supplemented with a trace amount of the holotoxin (LT) (0.02-20 ng), was examined for the adjuvant effect on antibody (Ab) responses against influenza inactivated haemagglutinin (HA) vaccine in Balb/c mice. Each mouse received a primary intranasal (i.n.) inoculation with the vaccine (1.5 mu g), prepared from PR8 (H1N1) virus, together with LT-containing LTB and in 4 weeks a second i.n. inoculation of the vaccine alone. The inoculation of the vaccine with the LT-containing LTB induced significantly high primary and secondary anti-HA IgA and IgG Ab responses in the nasal wash and the serum, while the vaccine with LTB or less than 2 ng of LT induced little response. The synergistic adjuvant effect was maximal in the concentration of LTB supplemented with 0.2-2 ng of LT. Under these conditions, the augmented IgA and IgG Ab responses, which are cross-protective to PR8 HA molecules, provided complete cross-protection against PR8 virus challenge in mice immunized with heterologous vaccine within the same subtype. These results suggest that LTB containing a trace amount of LT can be used as a potent adjuvant for nasal vaccination of humans against influenza.
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页码:1083 / 1089
页数:7
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