RAPID ACCURATE DETERMINATION OF ASCORBIC ACID IN BLOOD PLASMA

被引：19

作者：

HUBMANN, B

MONNIER, D

ROTH, M

机构：

[1] Laboratoire central, Hôpital cantonal de Genève, Institut de Chimie analytique

来源：

CLINICA CHIMICA ACTA | 1969年 / 25卷 / 01期

关键词：

D O I：

10.1016/0009-8981(69)90242-3

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

At pH 4, the non-fluorescent 1,2-naphthoquinone-4-sulfonic acid is quantitatively reducedby ascorbic acid to 1,2-dihydroxynaphthalene-4-sulfomc acid, which intensely fluoresces at λex = 330 nm and λf1= 465 nm. This permits to determine ascorbic acid in aqueous solution and in oxalated blood plasma. In the latter case, protein is removed by precipitation with metaphosphoric acid. When compared with other techniques of measuring the reducing power of vitamin C, this method provides far better sensitivity and shows good reproducibility (standard deviation ±1.8%). It is easy to perform and was found very satisfactory for the determination of ascorbic acid in the blood of patients having subnormal levels of this vitamin. The limit of sensitivity is 0.1 μg per ml of blood plasma. © 1969.

引用

页码：161 / &