EFFECTS OF CHRONIC ALCOHOL AND VITAMIN-E CONSUMPTION ON AGING PIGMENTS AND LEARNING-PERFORMANCE IN MICE

Chronic ethanol consumption further accelerates age-related impairment of shuttle box avoidance learning in mice. The hypothesis was tested that the behavioral impairment is a result of brain lipofuscin pigment deposition, which may be accelerated by ethanol consumption and prevented by the antioxidant effects of pharmacological doses of vitamin E. Feeding an ethanol-containing liquid diet for 5 months did not increase the lipofuscin content when compared with mice pair-fed a liquid diet containing isocaloric amounts of sucrose or standard solid laboratory food containing nutritionally adequate amounts of vitamin E. Supplementation of diets with vitamin E decreased brain lipofuscin content in all groups but failed to prevent the age- or ethanol-induced learning deficit. There was no effect of chronic ethanol consumption on brain weights, DNA, RNA, or protein content. It is concluded that the age-related impairment of avoidance learning is accelerated by chronic alcohol consumption. At the molecular level this acceleration is not caused by an increased brain lipofuscin deposition nor is it prevented by the antioxidant effects of vitamin E. © 1979.