HETEROGENEITY OF IMMUNOREACTIVE DYNORPHIN-B - LIKE MATERIAL IN HUMAN, RAT, RABBIT AND GUINEA-PIG HEART

被引：14

作者：

SPAMPINATO, S ^{[1
]}

CANOSSA, M ^{[1
]}

VENTURA, C ^{[1
]}

BACHETTI, T ^{[1
]}

VENTURINI, R ^{[1
]}

BASTAGLI, L ^{[1
]}

BERNARDI, P ^{[1
]}

FERRI, S ^{[1
]}

机构：

[1] UNIV BOLOGNA,INST MED PATHOL,I-40126 BOLOGNA,ITALY

来源：

LIFE SCIENCES | 1991年 / 48卷 / 06期

关键词：

D O I：

10.1016/0024-3205(91)90470-V

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Immunoreactive dynorphin B-like material (ir-dyn B) was detected in acetic acid extracts of human atrial specimens and of rat, rabbit and guinea-pig atria and ventricles by a validated radioimmunoassay. Levels were high in rabbit atrium (66.76 +/- 7.04 pmol/g) but lower and superimposable in human and rat atria (28.18 +/- 3.20 and 30.22 +/- 2.45 pmol/g, respectively). Gel permeation chromatography revealed ir-dyn B eluting close to column exclusion and in forms with an apparently higher molecular weight than authentic dyn B in human and rat samples. In contrast, almost all the immunoreactivity from rabbit and guinea-pig acetic extracts eluted as a single peak in the region of standard dyn B. Reverse-phase high performance liquid chromatography of the pooled gel chromatography fractions of this peak showed up a molecular form with the same retention time as authentic dyn B and a second minor peak of unknown immunoreactive material eluting three fractions earlier. Digestion with carboxypeptidase B excluded the hypothesis that this latter could be dyn B-Arg14. Therefore, it might be a metabolite of endogenous dyn B recognized by the antibody used in this study.

引用

页码：551 / 559

页数：9

共 26 条

[1]

CONE RI, 1983, J NEUROSCI, V3, P2146

[2] THE POSTTRANSLATIONAL PROCESSING OF PRODYNORPHIN IN THE RAT ANTERIOR-PITUITARY [J].