HYPERTENSION-INDUCED CHANGES OF PLATELET-DERIVED GROWTH-FACTOR RECEPTOR EXPRESSION IN RAT AORTA AND HEART

被引:67
作者
SARZANI, R
ARNALDI, G
CHOBANIAN, AV
机构
[1] BOSTON UNIV, SCH MED,WHITAKER CARDIOVASC INST,80 E CONCORD ST, BOSTON, MA 02118 USA
[2] BOSTON UNIV, SCH MED,DIV MED, BOSTON, MA 02118 USA
关键词
PLATELET-DERIVED GROWTH FACTOR; GROWTH; ESSENTIAL HYPERTENSION; AORTA; HEART;
D O I
10.1161/01.HYP.17.6.888
中图分类号
R6 [外科学];
学科分类号
1002 ; 100210 ;
摘要
Hypertension-associated growth of vascular smooth muscle cells might be mediated in vivo by platelet-derived growth factor (PDGF). Our previous investigations in hypertensive rats failed to demonstrate changes in aortic steady-state mRNA levels of PDGF A or B chains. The current studies were performed to determine whether hypertension might affect the expression of PDGF receptors. We studied PDGF alpha- and beta-receptor gene expression by Northern analysis using human and rat cDNA probes. Studies of tissue distribution revealed that PDGF beta-receptor mRNA was most abundant in total aorta and aortic media, whereas the PDGF alpha-receptor mRNA was most abundant in the lung and was expressed at low levels in aortic tissue. Deoxycortico-sterone acetate (DOCA)-salt hypertension induced a threefold increase in aortic steady-state PDGF beta-receptor mRNA levels. Aortic PDGF beta-receptor expression also was higher in spontaneously hypertensive rats (SHRs) when compared with age-matched normotensive Wistar-Kyoto (WKY) controls. Aortic PDGF alpha-receptor steady-state mRNA levels were unchanged in DOCA-salt hypertension and were expressed at similar levels in WKY rats and SHRs. Unlike the findings with aorta, cardiac PDGF beta- and alpha-receptor and PDGF B-chain expressions were unchanged in the DOCA-salt model and were decreased in SHRs. These findings indicate that hypertension can increase aortic steady-state mRNA levels for PDGF beta-receptor. They also indicate that tissue-specific expression of the genes of the PDGF ligand/receptor system are differentially regulated in hypertension.
引用
收藏
页码:888 / 895
页数:8
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