IDENTIFICATION OF NERVE GROWTH FACTOR-RESPONSIVE SEQUENCES WITHIN THE 5' REGION OF THE BOVINE PREPROTACHYKININ GENE

被引：30

作者：

GILCHRIST, CA ^{[1
]}

MORRISON, CF ^{[1
]}

CHAPMAN, KE ^{[1
]}

HARMAR, AJ ^{[1
]}

机构：

[1] ROYAL EDINBURGH & ASSOC HOSP,MRC,BRAIN METAB UNIT,EDINBURGH EH10 5HF,SCOTLAND

来源：

DNA AND CELL BIOLOGY | 1991年 / 10卷 / 10期

关键词：

D O I：

10.1089/dna.1991.10.743

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The production of substance P and the mRNA encoding its precursor (preprotachykinin, PPT) is regulated by nerve growth factor (NGF) in dorsal root ganglion (drg) neurons. To explore the mechanism by which NGF regulates the production of PPT mRNA, we have transfected PC12 cells and F11 cells with plasmids containing the bovine PPT promoter linked to the reporter gene chloramphenicol acetyltransferase (CAT). We have identified (i) functional elements within the PPT promoter which are necessary for expression in the absence of NGF and (ii) two separate regions, each of approximately 250 bp, which confer NGF responsiveness. Both regions contained a sequence element, similar to a known transcription factor binding site, which is present in several other NGF-regulated genes.

引用

页码：743 / 749

页数：7

共 36 条

[1] NEUROPEPTIDE-Y GENE-EXPRESSION IN PC12 CELLS AND ITS REGULATION BY NERVE GROWTH-FACTOR - A MODEL FOR DEVELOPMENTAL REGULATION [J].

ALLEN, JM ;

MARTIN, JB ;

HEINRICH, G .

MOLECULAR BRAIN RESEARCH, 1987, 3 (01) :39-43

[2] BUFFER GRADIENT GELS AND S-35 LABEL AS AN AID TO RAPID DNA-SEQUENCE DETERMINATION [J].

BIGGIN, MD ;

GIBSON, TJ ;

HONG, GF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (13) :3963-3965

[3] STRUCTURE AND METHYLATION OF THE HUMAN CALCITONIN ALPHA-CGRP GENE [J].

BROAD, PM ;

SYMES, AJ ;

THAKKER, RV ;

CRAIG, RK .

NUCLEIC ACIDS RESEARCH, 1989, 17 (17) :6999-7011

[4] FUNCTIONAL-ANALYSIS OF THE TRANSCRIPTION CONTROL REGION LOCATED WITHIN THE AVIAN RETROVIRAL LONG TERMINAL REPEAT [J].

CULLEN, BR ;

RAYMOND, K ;

JU, G .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (03) :438-447

[5] THYROID OR GLUCOCORTICOID HORMONE INDUCES PRE-GROWTH-HORMONE MESSENGER-RNA AND ITS PROBABLE NUCLEAR PRECURSOR IN RAT PITUITARY-CELLS [J].

DOBNER, PR ;

KAWASAKI, ES ;

YU, LY ;

BANCROFT, FC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (04) :2230-2234

[6] NEUROCHEMICAL CHARACTERISTICS OF A NOVEL DORSAL-ROOT GANGLION X NEUROBLASTOMA HYBRID CELL-LINE, F-11 [J].

FRANCEL, PC ;

HARRIS, K ;

SMITH, M ;

FISHMAN, MC ;

DAWSON, G ;

MILLER, RJ .

JOURNAL OF NEUROCHEMISTRY, 1987, 48 (05) :1624-1631

[7] NERVE GROWTH-FACTOR REGULATES TYROSINE-HYDROXYLASE GENE-TRANSCRIPTION THROUGH A NUCLEOPROTEIN COMPLEX THAT CONTAINS C-FOS [J].

GIZANGGINSBERG, E ;

ZIFF, EB .

GENES & DEVELOPMENT, 1990, 4 (04) :477-491

[8] THE ROUS-SARCOMA VIRUS LONG TERMINAL REPEAT IS A STRONG PROMOTER WHEN INTRODUCED INTO A VARIETY OF EUKARYOTIC CELLS BY DNA-MEDIATED TRANSFECTION [J].

GORMAN, CM ;

MERLINO, GT ;

WILLINGHAM, MC ;

PASTAN, I ;

HOWARD, BH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (22) :6777-6781

[9]

GREENBERG ME, 1985, J BIOL CHEM, V260, P4101

[10] ESTABLISHMENT OF A NORADRENERGIC CLONAL LINE OF RAT ADRENAL PHEOCHROMOCYTOMA CELLS WHICH RESPOND TO NERVE GROWTH-FACTOR [J].

GREENE, LA ;

TISCHLER, AS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (07) :2424-2428

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