BISSULFOSUCCINIMIDYL ESTERS OF ALIPHATIC DICARBOXYLIC-ACIDS - A NEW CLASS OF AFFINITY DIRECTED BETA-BETA CROSS-LINKERS OF HBA

被引：5

作者：

MANJULA, BN ^{[1
]}

ROY, RP ^{[1
]}

SMITH, PK ^{[1
]}

ACHARYA, AS ^{[1
]}

机构：

[1] BIOAFFIN SYST INC,ROSCOE,IL

来源：

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY | 1994年 / 22卷 / 03期

关键词：

D O I：

10.3109/10731199409117907

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The potential of sulfosuccinimidyl ester of suberic acid as an intramolecular crosslinker of HbG directed to the positive charge rich domain of the protein (DPG pocket) has been investigated. The suberate ester introduced crosslinks between the beta-chains and inhibited the dissociation of HbA into alpha beta dimers. The facile crosslinking reaction seen with this suberate ester as compared to the absence of crosslinking with the diaspirin derivative of suberic acid suggests the strong 'steering' influence of the sulfosuccinimidyl moiety of the reagent to the targeted site. The application of this crosslinking approach in the preparation of Hb based blood substitutes is discussed.

引用

页码：747 / 752

页数：6

共 10 条

[1]

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[2]

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[3]

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[4]

ACHARYA AS, 1985, J BIOL CHEM, V260, P6039