EFFECT OF PHENETHYL ALCOHOL ON CELL CULTURE GROWTH .2. ISOLATED CELL COMPONENTS AND LYSOSOMAL ENZYMES

Treatment of isolated chromosomes or nuclei with phenethyl alcohol was without effect on staining intensity and stain distribution, which is in contrast to the condition found when whole cells were treated with phenethyl alcohol. Isolated nuclei labeled with radioactive thymidine did not lose radioactivity when treated with phenethyl alcohol. Phenethyl alcohol treatment of either isolated lysosomes or whole cells released lysosomal enzymes which could account for the loss of DNA from cell cultures. Phenethyl alcohol treatment reduced the ability of cells to accumulate uridine and this reduction was reflected in a decreased incorporation of uridine. © 1969.