DETERMINATION OF DEBRISOQUINE AND 4-HYDROXYDEBRISOQUINE IN HUMAN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND FLUORESCENCE DETECTION

被引：7

作者：

HEMPENIUS, J ^{[1
]}

STAAL, B ^{[1
]}

JEURING, HJ ^{[1
]}

MENSINK, CK ^{[1
]}

JONKMAN, JHG ^{[1
]}

机构：

[1] STATE UNIV GRONINGEN,DEPT ANALYT CHEM & TOXICOL,9714 AW GRONINGEN,NETHERLANDS

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1990年 / 13卷 / 08期

关键词：

D O I：

10.1080/01483919008048980

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid, simple and selective method for the determination of debrisoquine and 4-hydroxydebrisoquine in human urine is described. The sample preparation was done by direct injection of urine after dilution with water. Separation and quantitation was performed by reversed phase high performance liquid chromatography with fluorescense detection. The lower limits of quantitation for debrisoquine and 4-hydroxydebrisoquine were 0.25 mg.L-1 using 200 μL of urine sample. The intra-day reproducibility was about 3.5 % or better for both components. The inter-day reproducibility was 4.5 % or better for both compounds. © 1990, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：1627 / 1636

页数：10

共 9 条

[1]

ALLEN JG, 1975, DRUG METAB DISPOS, V3, P332

[2] GAS-LIQUID-CHROMATOGRAPHIC ELECTRON-CAPTURE DETERMINATION OF SOME MONOSUBSTITUTED GUANIDO-CONTAINING DRUGS [J].

ERDTMANSKY, P ;

GOEHL, TJ .

ANALYTICAL CHEMISTRY, 1975, 47 (04) :750-752

[3] DETERMINATION OF DEBRISOQUINE AND ITS 4-HYDROXY METABOLITE IN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

HARRISON, PM ;

TONKIN, AM ;

DIXON, ST ;

MCLEAN, AJ .

JOURNAL OF CHROMATOGRAPHY, 1986, 374 (01) :204-208

[4] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAYS FOR BUFURALOL 1'-HYDROXYLASE, DEBRISOQUINE 4-HYDROXYLASE, AND DEXTROMETHORPHAN O-DEMETHYLASE IN MICROSOMES AND PURIFIED CYTOCHROME-P-450 ISOZYMES OF HUMAN-LIVER [J].

KRONBACH, T ;

MATHYS, D ;

GUT, J ;

CATIN, T ;

MEYER, UA .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :24-32

[5] DETERMINATION OF DEBRISOQUINE AND ITS 4-HYDROXY METABOLITE IN BIOLOGICAL-FLUIDS BY GAS-CHROMATOGRAPHY WITH FLAME-IONIZATION AND NITROGEN-SELECTIVE DETECTION [J].

LENNARD, MS ;

SILAS, JH ;

SMITH, AJ ;

TUCKER, GT .

JOURNAL OF CHROMATOGRAPHY, 1977, 133 (01) :161-166

[6]

Massart D. L., 1988, CHEMOMETRICS TXB

[7] ASSAY OF DEBRISOQUINE AND 4-HYDROXYDEBRISOQUINE IN URINE BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING ONLINE SAMPLE CLEANUP ON A STANDARD ISOCRATIC CHROMATOGRAPH [J].

MONCRIEFF, J .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1988, 428 (01) :178-182

[8]

STEINER E, 1987, THESIS STOCKHOLM

[9] LIQUID-CHROMATOGRAPHIC ASSAY FOR DEBRISOQUINE AND 4-HYDROXYDEBRISOQUINE IN URINE [J].

WESTWOOD, BE ;

HARMAN, PJ ;

MASHFORD, ML .

JOURNAL OF CHROMATOGRAPHY, 1986, 374 (01) :200-203

← 1 →