CDNA CLONING OF A NOVEL CDC2+/CDC28-RELATED PROTEIN-KINASE FROM RICE

被引：64

作者：

HATA, S

机构：

[1] National Institute of Agrobiological Resources, Tsukuba, Ibaraki, 305

来源：

FEBS LETTERS | 1991年 / 279卷 / 01期

关键词：

SERINE THREONINE KINASE; CDC2+; CDC28; CDNA CLONING; ORYZA-SATIVA;

D O I：

10.1016/0014-5793(91)80271-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone, named R2, has been isolated by screening a rice cell cDNA library with a redundant oligonucleotide probe derived from the conserved ATP binding site of cdc2+/CDC28 protein kinases. The cDNA contained the entire coding sequence for a 424 amino acid polypeptide with a molecular mass of 47.6 kDa. The R2 mRNA, 2.1 kb in size, was expressed in both cultured rice cells and rice seedlings at similar levels. The predicted R2 protein has canonical motifs for ATP binding and catalysis, and is significantly homologous (up to 47%) to members of the cdc2+/CDC28 subfamily of serine/threonine protein kinase. The R2 protein is a novel member of the subfamily.

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页码：149 / 152

页数：4

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