A FUNGAL MATING TYPE PROTEIN THAT REGULATES SEXUAL AND ASEXUAL DEVELOPMENT CONTAINS A POU-RELATED DOMAIN

被引:59
作者
TYMON, AM
KUES, U
RICHARDSON, WVJ
CASSELTON, LA
机构
[1] QUEEN MARY & WESTFIELD COLL, SCH BIOL SCI, LONDON E1 4NS, ENGLAND
[2] UNIV OXFORD, DEPT PLANT SCI, OXFORD 0X1 3RB, ENGLAND
关键词
A MATING TYPE PROTEIN; COPRINUS-CINEREUS; POUS LIKE DOMAIN COPS; SEXUAL-ASEXUAL DEVELOPMENT; TRANSCRIPTION FACTOR;
D O I
10.1002/j.1460-2075.1992.tb05232.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The A mating type factor of the fungus Coprinus cinereus regulates essential steps in sexual development. Here we describe features of one of the four specificity genes of the A 42 factor. By transformation we show that the gene regulates not only sexual development but also asexual sporulation. DNA sequence analysis shows that the gene beta-1-1, encodes a protein with a DNA binding motif and is thus likely to be a transcription factor. The DNA binding domain is an unusual homeodomain with D replacing the normally invariant N in the recognition helix and apparent absence of helix II. The homeodomain is linked to a helical region related to the POUs domain, which is part of a bipartite DNA binding domain of certain animal transcription factors. Like POU factors, the beta-1-1 protein has regions rich in serine, threonine and proline which are possible transactivation domains. Putative dimerization domains and sites for post-translational modification are described.
引用
收藏
页码:1805 / 1813
页数:9
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