CLONING AND SEQUENCE-ANALYSIS OF A CDNA-ENCODING THE ALPHA-SUBUNIT OF MOUSE BETA-N-ACETYLHEXOSAMINIDASE AND COMPARISON WITH THE HUMAN ENZYME

被引：41

作者：

BECCARI, T

HOADE, J

ORLACCHIO, A

STIRLING, JL

机构：

[1] KINGS COLL LONDON,DIV BIOMOLEC SCI,KENSINGTON CAMPUS,CAMPDEN HILL,LONDON W8 7AH,ENGLAND

[2] UNIV PERUGIA,DIPARTIMENTO MED SPERIMENTAL & SCI BIOCHIM,I-06100 PERUGIA,ITALY

来源：

BIOCHEMICAL JOURNAL | 1992年 / 285卷

关键词：

D O I：

10.1042/bj2850593

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

cDNAs encoding the mouse beta-N-acetylhexosaminidase alpha-subunit were isolated from a mouse testis library. The longest of these (1.7 kb) was sequenced and showed 83% similarity with the human alpha-subunit cDNA sequence. The 5' end of the coding sequence was obtained from a genomic DNA clone. Alignment of the human and mouse sequences showed that all three putative N-glycosylation sites are conserved, but that the mouse alpha-subunit has an additional site towards the C-terminus. All eight cysteines in the human sequence are conserved in the mouse. There are an additional two cysteines in the mouse alpha-subunit signal peptide. All amino acids affected in Tay-Sachs-disease mutations are conserved in the mouse.

引用

页码：593 / 596

页数：4

共 29 条

[1] 7 NOVEL TAY-SACHS MUTATIONS DETECTED BY CHEMICAL MISMATCH CLEAVAGE OF PCR-AMPLIFIED CDNA FRAGMENTS [J].