EFFICIENT TRANSFORMATION OF TOBACCO BY ULTRASONICATION

被引：40

作者：

ZHANG, LJ ^{[1
]}

CHENG, LM ^{[1
]}

XU, N ^{[1
]}

ZHAO, NM ^{[1
]}

LI, CG ^{[1
]}

YUAN, J ^{[1
]}

JIA, SR ^{[1
]}

机构：

[1] TSING HUA UNIV, DEPT BIOL SCI & BIOTECHNOL, BEIJING 100084, PEOPLES R CHINA

来源：

BIO-TECHNOLOGY | 1991年 / 9卷 / 10期

关键词：

D O I：

10.1038/nbt1091-996

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We report a simple, direct gene transfer technique based on sonication. Transient expression of a beta-glucoronidase (GUS) gene was detected in 86 percent of treated leaf segments of Nicotiana tabacum, and the area of transformed tissue on these segments reached 60-70 percent. GUS activity in the leaves of regenerated, kanamycin resistant plantlets was found at a frequency of 22 percent, and analysis of R1 regenerated seedlings showed that both kanamycin resistance and GUS behaved as typical linked dominant genes.

引用

页码：996 / 997

页数：2

共 6 条

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[3] JOERSBO M, 1990, PLANT CELL REP, V9, P207, DOI 10.1007/BF00232181
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[5] UPTAKE AND TRANSIENT EXPRESSION OF CHIMERIC GENES IN SEED-DERIVED EMBRYOS
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[6] XU N, 1990, ACTA BIOPHYSICA SINI, V6, P281

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