SEQUENCE OF THE GLN1 GENE OF SACCHAROMYCES-CEREVISIAE - ROLE OF THE UPSTREAM REGION IN REGULATION OF GLUTAMINE-SYNTHETASE EXPRESSION

被引：59

作者：

MINEHART, PL ^{[1
]}

MAGASANIK, B ^{[1
]}

机构：

[1] MIT,DEPT BIOL,CAMBRIDGE,MA 02139

来源：

JOURNAL OF BACTERIOLOGY | 1992年 / 174卷 / 06期

关键词：

D O I：

10.1128/jb.174.6.1828-1836.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The GLN1 gene, encoding glutamine synthetase in Saccharomyces cerevisiae, was sequenced, and its encoded polypeptide was shown to have significant homology to other eukaryotic glutamine synthetases. S1 analysis has defined the transcriptional start site of the gene. Upstream analysis of the gene using lacZ fusions has verified transcriptional control of the gene and has identified a nitrogen upstream activation sequence which is required for the increased transcription of GLN1 seen when glutamine is replaced by glutamate as the nitrogen source. cis-acting sites required for the increased transcription in response to purine starvation also have been localized.

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页码：1828 / 1836

页数：9

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