THIOREDOXIN REDUCTION DEPENDENT ON ALPHA-KETOACID OXIDATION BY ALPHA-KETOACID DEHYDROGENASE COMPLEXES

被引：36

作者：

BUNIK, V ^{[1
]}

FOLLMANN, H ^{[1
]}

机构：

[1] UNIV KASSEL, FACHBEREICH BIOL CHEM, D-34109 KASSEL, GERMANY

来源：

FEBS LETTERS | 1993年 / 336卷 / 02期

关键词：

THIOREDOXIN; THIOREDOXIN REDUCTASE; ALPHA-KETOACID DEHYDROGENASE COMPLEX; INSULIN; LIPOATE; MITOCHONDRION;

D O I：

10.1016/0014-5793(93)80801-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The pyruvate and alpha-ketoglutarate dehydrogenase complexes isolated from pig heart mitochondria promote the reduction of thioredoxin in the presence of their alpha-ketoacid substrates, coenzyme A, and free lipoate. Substrate-specific generation of reduced thioredoxin was established by two independent methods, viz. reduction of insulin and thioredoxin reductase-catalyzed NADPH formation. Dihydrolipoate accumulating in the absence of NAD+ is the likely intermediate. A redox function in alpha-ketoacid oxidation provides a potential role for the specific thioredoxins previously identified by us in mitochondria.

引用

页码：197 / 200

页数：4

共 12 条

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