RECOMBINANT WHOLE CELLS AS CATALYSTS FOR THE ENZYMATIC-SYNTHESIS OF OLIGOSACCHARIDES AND GLYCOPEPTIDES

被引：23

作者：

HERRMANN, GF ^{[1
]}

WANG, P ^{[1
]}

SHEN, GJ ^{[1
]}

WONG, CH ^{[1
]}

机构：

[1] Scripps Res Inst, RES INST, DEPT CHEM, LA JOLLA, CA 92037 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 1994年 / 33卷 / 12期

关键词：

D O I：

10.1002/anie.199412411

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An easier, faster, cheaper approach to the enzymatic synthesis of oligosaccharides would be to employ whole cells rather than purified enzymes. In this report recombinant E. coli cells mediate the mannosylation of monosaccharides and glycopeptides. It was also proven that the relevant enzyme, mannosyl transferase, does not leak from the periplasmic space of the cells into the medium. Copyright © 1994 by VCH Verlagsgesellschaft mbH, Germany

引用

页码：1241 / 1242

页数：2

共 31 条

[1] SYSTEMATIC-APPROACH TO THE ANALYSIS OF C-13 NMR-SPECTRA OF COMPLEX CARBOHYDRATES .1. ALPHA-D-MANNOPYRANOSYL RESIDUES IN OLIGOSACCHARIDES AND THEIR IMPLICATIONS FOR STUDIES OF GLYCOPROTEINS AND GLYCOPEPTIDES
ALLERHAND, A
BERMAN, E
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1984, 106 (08) : 2400 - 2412
[2] ANKEL H, 1970, J BIOL CHEM, V245, P3945
[3] THE USE OF AN IMMOBILIZED CYCLIC MULTIENZYME SYSTEM TO SYNTHESIZE BRANCHED PENTA-SACCHARIDES AND HEXA-SACCHARIDES ASSOCIATED WITH BLOOD-GROUP I EPITOPES
AUGE, C
MATHIEU, C
MERIENNE, C
[J]. CARBOHYDRATE RESEARCH, 1986, 151 : 147 - 156
[4] ENZYMATIC-SYNTHESIS OF DISACCHARIDE-SERINE AND PEPTIDE CONJUGATES
BAY, S
NAMANE, A
CANTACUZENE, D
[J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1993, 3 (12) : 2515 - 2520
[5] Charney W., 1967, MICROBIAL TRANSFORMA
[6] BAKERS-YEAST MEDIATED TRANSFORMATIONS IN ORGANIC-CHEMISTRY
CSUK, R
GLANZER, BI
[J]. CHEMICAL REVIEWS, 1991, 91 (01) : 49 - 97
[7] ENZYMATIC METHODS IN PREPARATIVE CARBOHYDRATE-CHEMISTRY
DAVID, S
AUGE, C
GAUTHERON, C
[J]. ADVANCES IN CARBOHYDRATE CHEMISTRY AND BIOCHEMISTRY, 1991, 49 : 175 - 237
[8] GILLESPIE W, 1992, J BIOL CHEM, V267, P21004
[9] NUCLEOSIDE PHOSPHATE SUGARS - SYNTHESES ON PRACTICAL SCALES FOR USE AS REAGENTS IN THE ENZYMATIC PREPARATION OF OLIGOSACCHARIDES AND GLYCOCONJUGATES
HEIDLAS, JE
WILLIAMS, KW
WHITESIDES, GM
[J]. ACCOUNTS OF CHEMICAL RESEARCH, 1992, 25 (07) : 307 - 314
[10] HERRMANN GF, 1993, ANGEW CHEM INT EDIT, V32, P1342, DOI 10.1002/anie.199313421

← 1 2 3 4 →