TRANS-GALACTOSYLATION ACTIVITY OF SWEET ALMOND ALPHA-GALACTOSIDASE - SYNTHESIS OF SACCHARIDES

被引：22

作者：

DEY, PM

机构：

[1] Department of Biochemistry, Royal Holloway College, University of London, Egham, Surrey TW20 OEX, Egham Hill

来源：

PHYTOCHEMISTRY | 1979年 / 18卷 / 01期

关键词：

inhibition; Prunus dulcis; Rosaceae; sweet almond; transgalactosylation; α-galactosidase;

D O I：

10.1016/S0031-9422(00)90910-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sweet almond α-galactosidase (α-d-galactoside galactohydrolase, EC 3.2.1.22) catalyses hydrolytic, synthetic (de novo) and transfer reactions. Transfer products were formed using p-nitrophenyl α-d-galactoside as the galactosyl donor and glucose, galactose, sucrose, maltose and lactose as acceptors; several of the products were identified. The enzyme also caused elongation of the oligosaccharide chain of two substrates (melibiose and raffinose). In addition, the enzyme catalysed condensation of free galactose, yielding oligosaccharides. The products were identified in all cases by chromatography. © 1979.

引用

页码：35 / 38

页数：4

共 34 条

[1]

ANAGNOSTOPOULOS C, 1955, Arch Sci Biol (Bologna), V39, P631

[2]

ANAGNOSTOPOULOS C, 1954, B SOC CHIM BIOL, V36, P1115

[3]

BACON JSD, 1950, ARCH BIOCHEM, V28, P467

[4] STUDIES OF ASPERGILLUS-NIGER .1. THE STRUCTURE OF THE POLYGLUCOSAN SYNTHESISED BY ASPERGILLUS-NIGER 152 [J].