DEVELOPMENT OF A STIRRED TANK REACTOR SYSTEM FOR THE PRODUCTION OF LIGNIN PEROXIDASES (LIGNINASES) BY PHANEROCHAETE-CHRYSOSPORIUM BKM-F-1767

被引：35

作者：

MICHEL, FC ^{[1
]}

GRULKE, EA ^{[1
]}

REDDY, CA ^{[1
]}

机构：

[1] MICHIGAN STATE UNIV,DEPT CHEM ENGN,E LANSING,MI 48824

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY | 1990年 / 5卷 / 2-3期

关键词：

Filamentous fungi; Fungal pellets; Ligninase production; Submerged culture; White-rot fungus;

D O I：

10.1007/BF01573859

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lignin peroxidases produced by Phanerochaete chrysosporium have several important potential industrial applications based on their ability to degrade lignin and lignin-like compounds. A stirred tank reactor system for the production of lignin peroxidases is described here. Included in this study is an examination of the mechanics of pellet biocatalyst formation and the optimization of an acetate buffered medium. Higher levels of lignin peroxidase were obtained with acetate buffer compared to the other buffer systems tested. Concentrations of 0.05% (w/v) Tween 80 and 0.4 mM veratryl alcohol gave optimal lignin peroxidase activity in acetate buffered medium. In shake flask cultures, mycelial fragments in the inoculum aggregated into pellets during the first eight hours of incubation and thereafter increased in size through the eighth day. The agitation rate in shake flask cultures affected pellet size, the number of pellets formed, and lignin peroxidase activity. Transfer of fungal pellets from shake flask culture to a continuously oxygenated baffled stirred tank reactor (STR) resulted in production of high lignin peroxidase titres comparable to those of shake flask cultures when the agitation rate, oxygen dispersion and foaming were closely controlled. © 1990 Society for Industrial Microbiology.

引用

页码：103 / 112

页数：10

共 19 条

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