A SIMPLE AND RAPID TECHNIQUE FOR IDENTIFICATION OF LARGE NUMBERS OF INDIVIDUAL MOSQUITOS USING DNA HYBRIDIZATION

被引：12

作者：

COCKBURN, AF

机构：

[1] USDA, ARS, Insects Affecting Man and Animals Research Laboratory, Gainesville, Florida

来源：

ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY | 1990年 / 14卷 / 03期

关键词：

Anopheles quadrimaculatus; DNA probes; sibling species;

D O I：

10.1002/arch.940140306

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A general method for obtaining species‐specific repetitive DNA sequences is described. The method is based on the detection of recombinant DNA clones containing repetitive sequences using labeled total genomic DNA. These repetitive DNA sequences can be used to identify individual mosquito adults, pupae, and larvae squashed on filter membranes (squash blots). This technique was used to distinguish individuals of the four sibling species of the Anopheles quadrimaculatus complex. Repetitive DNA sequences and squash blots can be of use for rapid identification of other insect species in field collections. Copyright © 1990 Wiley‐Liss, Inc.

引用

页码：191 / 199

页数：9

共 10 条

[1] REPETITIVE DNA INTERSPERSION PATTERNS IN DIPTERA [J].