IMMUNOHISTOCHEMICAL ASSESSMENT OF CELLULAR PROLIFERATION IN THE DEVELOPING HUMAN CNS USING FORMALIN-FIXED PARAFFIN-EMBEDDED MATERIAL

被引：22

作者：

MOLLGARD, K ^{[1
]}

SCHUMACHER, U ^{[1
]}

机构：

[1] UNIV SOUTHAMPTON,SOUTHAMPTON SO9 3TU,HANTS,ENGLAND

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1993年 / 46卷 / 03期

关键词：

CNS DEVELOPMENT; PROLIFERATING CELL NUCLEAR ANTIGEN; PARAFFIN-EMBEDDED MATERIAL; (HUMAN);

D O I：

10.1016/0165-0270(93)90066-Z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Assessment of cell proliferation in the developing human brain has been limited to counting mitotic cells, since the other procedures currently used for determination of the mitotic activity either depend on experimental manipulation of the tissue, e.g., labeling with radioactive thymidine precursors or with bromodeoxyuridine, or on frozen sections of the tissue, e.g., Ki-67 immunoreactivity. As most of the developing human brain material available is formalin fixed and paraffin embedded, these procedures are not practical. An immunohistochemical technique based on a monoclonal antibody directed against a proliferating cell nuclear antigen (PCNA) was used for detecting cellular proliferation in the developing human brain. Known progenitor cells in proliferating zones showed distinct PCNA-reactivity, but the method also revealed new areas of cell proliferation in fixed paraffin-embedded tissue blocks from the developing human brain.

引用

页码：191 / 196

页数：6

共 9 条

[1] PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) IMMUNOLOCALIZATION IN PARAFFIN SECTIONS - AN INDEX OF CELL-PROLIFERATION WITH EVIDENCE OF DEREGULATED EXPRESSION IN SOME NEOPLASMS [J].