EXPRESSION OF ADHESION MOLECULES CD11/CD18 (LEU-CAMS, BETA-2-INTEGRINS), CD54 (ICAM-1) AND CD58 (LFA-3) IN B-CHRONIC LYMPHOCYTIC-LEUKEMIA

被引：24

作者：

KIMBY, E

RINCON, J

PATARROYO, M

MELLSTEDT, H

机构：

[1] Department of Medicine, Division of Hematology, Danderyd Hospital, Stockholm

[2] Department of Immunologi, Karolinska Institute, Stockholm

[3] Department of Oncology (Radiumhemmet), Karolinska Hospital, Stockholm

来源：

LEUKEMIA & LYMPHOMA | 1994年 / 13卷 / 3-4期

关键词：

ADHESION MOLECULES; CD11/18; CD54; ICAM-1; CD58; LFA-3; B-CLL; MONOCLONAL; B LYMPHOCYTES;

D O I：

10.3109/10428199409056294

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Cell adhesion molecules (CAMs) are cell surface proteins with unique specificities that allow intercellular adhesion. The importance of CAMs for normal lymphocyte growth and differentiation is underscored by the association between neoplastic disease states and abnormal CAM expression. In the present study we analysed the cell surface expression of several CAMs on peripheral blood lymphocytes from patients with progressive chronic lymphocytic leukemia of B-cell type (B-CLL) (n = 21) and stable monoclonal B-lymphocytosis of undetermined significance (B-MLUS) (n = 20). The CAM expression was analysed on the B-cell clone and on normal T- and NK-cell populations separately using monoclonal antibodies (MAbs). A phorbol ester-induced lymphocyte aggregation assay and blocking MAbs were also used. The B-cell clone in B-CLL expressed ICAM-1 (CD54) more frequently and at a higher density than in B-MLUS. The brightest CD54 expression was noted in patients with prominent lymphadenopathy and/or splenomegaly. The beta 2 integrin CD11a (Leu-CAMa, LFA-1) was detected on some B-cell clones and seemed to relate to tissue localization of the disease. T and NK cells showed a low expression of CD11a in B-CLL patients, while in B-MLUS a high proportion of non-clonal cells coexpressed CD11a with a high staining intensity. The relative numbers of both CD18+ as well as CD2+ cells showed a positive correlation with phorbol ester induced cell aggregation in B-MLUS patients (p < 0.05). The aggregation was blocked by adding MAbs against CD18 in most cases but to a greater extent in B-CLL. These results extend and corroborate our earlier findings on surface phenotypic characteristics of clonal and non-clonal lymphocytes in different clinical subtypes of B-CLL. CAM expression on the monoclonal lymphocytes may play a role in their interaction with regulatory immune cells and their tissue localization.

引用

页码：297 / 306

页数：10

共 32 条

[1] Springer T.A., Adhesion receptors of the immune system, Nature, 346, pp. 425-434, (1990)
[2] Patanoyo M., Leukocyte adhesion in host defence and tissue injury, Clin. Immunol. Immunopath., 60, pp. 333-348, (1991)
[3] Patarroyo M., Clark E.A., Prieto J., Kantor C., Gahmberg C.G., Identification of a novel adhesion molecule in human leukocytes by monoclonal antibody LB-2, FEBS Lett., 210, pp. 127-131, (1987)
[4] Patarroyo M., Prieto J., Ernberg I., Gahmberg C.G., Absence, or low expression, of leukocyte adhesion molecules CD11 and CD 18 on Burkitt lymphoma cells, Int. J. Cancer, 41, pp. 901-907, (1988)
[5] Clayberger C., Medeiros I.J., Link M.P., Warnke R.A., Wright A., Koller T.D., Smith S.D., Krensky A.M., Absence of cell surface LFA-1 as a mechanism of escape from immunosurveillance, Lancet, ii, pp. 533-536, (1987)
[6] Gregory C.D., Murray R.J., Edwards C.F., Rick-Inson A.B., Downregulation of cell adhesion molecules LFA-3 and ICAM-1 in Epstein-Barr virus-positive Burkitt's lymphoma underlies tumor cell escape from virus-specific T cell surveillance, J. Exp. Med., 167, pp. 1811-1824, (1988)
[7] Maio M., Pinto A., Carbone A., Zagonel V., Gloghini A., Marotta G., Cirillo D., Colombatti A., Ferrara F., del Vrchio L., Ferrone S., Differential expression of CD54/intercellular adhesion molecule-1 in myeloid leuke-mias and in lymphoproliferative disorders, Blood, 76, pp. 783-790, (1990)
[8] Reichert R.A., Gallatin M., Weissman I.L., Butcher E.C., Germinal center B cells lack homing receptors necessary for normal lymphocyte recirculation, J. Exp. Med., 157, pp. 813-827, (1983)
[9] Jalkanen S., Reichert R.A., Gallatin W.M., Bargatze R.F., Weissman I.L., Butcher E.C., Homing receptors and the control of lymphocyte migration, Immunol. Rev., 91, pp. 39-60, (1986)
[10] Horst E., Radaszkiewicz T., Hooftman-den Otter A., Pie-Ters R., ban Dongen J.J.M., Meijer J.L.M., Pals S.T., Expression of the leucocyte integrin LFA-1 (CD11a/CD 18) and its ligand ICAM-1 (CD54) in relation to lineage derivation and stage of differentiation but not to tumor grade, Leukemia, 10, pp. 848-853, (1991)

← 1 2 3 4 →