SYNTHESIS OF BETA-MERCAPTOETHYL-GLYCOSIDES BY ENZYMATIC REVERSE HYDROLYSIS AND TRANSGLYCOSYLATION

被引：9

作者：

DINTINGER, T ^{[1
]}

DUTHEILBOUEDEC, D ^{[1
]}

BOUCHONNEAU, M ^{[1
]}

TELLIER, C ^{[1
]}

机构：

[1] FAC SCI & TECHN NANTES,LAB RMN & REACTIV CHIM,CNRS,URA 472,F-44072 NANTES 03,FRANCE

来源：

BIOTECHNOLOGY LETTERS | 1994年 / 16卷 / 07期

关键词：

D O I：

10.1007/BF00136472

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The synthetic potential of Almond beta-glucosidase and Jack bean alpha-mannosidase in the presence of high amounts of beta-mercaptoethanol as glycosyl acceptor for the synthesis of beta-mercaptoethyl-glycosides was studied. The regioselectivity, O-glycosylation and/or S-glycosylation, and the stereoselectivity were analyzed with the reverse hydrolysis and the transglycosylation methods. With both enzymes, high yields of condensation are obtained without the use of chemical protective groups.

引用

页码：689 / 692

页数：4

共 5 条

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