MICRONUCLEUS TEST AND METAPHASE ANALYSES IN MICE EXPOSED TO KNOWN AND SUSPECTED SPINDLE POISONS

被引：42

作者：

MARRAZZINI, A ^{[1
]}

BETTI, C ^{[1
]}

BERNACCHI, F ^{[1
]}

BARRAI, I ^{[1
]}

BARALE, R ^{[1
]}

机构：

[1] UNIV FERRARA, DIPARTIMENTO BIOL EVOLUT, I-44100 FERRARA, ITALY

来源：

MUTAGENESIS | 1994年 / 9卷 / 06期

关键词：

D O I：

10.1093/mutage/9.6.505

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Micronucleus (Mn) and metaphase chromosome analyses were performed in mouse bone marrow cells with two known and eight suspected mitotic spindle poisons. Polychromatic (PCEs) and normochromatic (NCEs) erythrocytes were scored for presence of Mn, while structural (CAs) and numerical chromosome aberrations (NCAs), i.e. hyperploid cells, were evaluated by metaphase analysis. CAs were scored in first, and NCAs in the second metaphases, identified by BrdUrd differential staining. Hydroquinone induced Mn, NCAs and CAs; colchicine, vinblastine and, to a lesser extent, chloral hydrate, diazepam and econazole induced both Mn and NCAs; cadmium chloride and thimerosal induced Mn and CAs, while pyrimethamine and thiabendazole induced Mn only. The proposed stepwise protocol allowed satisfactory statistical evaluation of the effects induced with a reduction in the number of animals killed. An acceptable agreement was found between induction of Mn and NCAs, suggesting a possible use of the Mn test for revealing compounds with aneugenic properties.

引用

页码：505 / 515

页数：11

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