TRANSFORMING GROWTH-FACTOR-BETA INHIBITS SURFACTANT PROTEIN-A EXPRESSION INVITRO

被引：48

作者：

WHITSETT, JA ^{[1
]}

BUDDEN, A ^{[1
]}

HULL, WM ^{[1
]}

CLARK, JC ^{[1
]}

OREILLY, MA ^{[1
]}

机构：

[1] CHILDRENS HOSP MED CTR,DIV PULM BIOL,CINCINNATI,OH 45229

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1123卷 / 03期

关键词：

TRANSFORMING GROWTH FACTOR; SURFACTANT PROTEIN; (HUMAN CELL LINE);

D O I：

10.1016/0005-2760(92)90004-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Effects of members of the transforming growth factor-beta (TGF-beta) family on expression of surfactant protein A (SP-A) were determined in human pulmonary adenocarcinoma cells. TGF-beta decreased SP-A content in two distinct pulmonary adenocarcinoma cell lines with bronchiolar (NCI-H441-4) and alveolar (NCI-H820) cell characteristics. TGF-beta-1, beta-2 and beta-3 were equally effective in decreasing SP-A. Effects of the TGF-beta's on SP-A content were dose dependent, EC50 approximately 20-30 pg/ml for each form of TGF-beta. TGF-beta decreased cellular SP-A content in association with decreased levels of SP-A mRNA. Inhibitory effects of TGF-beta-1 on SP-A mRNA was time dependent, reaching maximal effects within 12-24 h, after which SP-A mRNA was approximately 10% of that present in untreated cells. Maximal inhibition of SP-A mRNA was observed at 250 pg/ml TGF-beta-1. TGF-beta-dependent inhibition of SP-A expression was not associated with altered cell morphology, growth, or viability. TGF-beta family members act directly on pulmonary adenocarcinoma cells to inhibit SP-A expression by mechanisms which are mediated, at least in part, at a pretranslational level.

引用

页码：257 / 262

页数：6

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