HUMAN DEOXYHEMOGLOBIN-2,3-DIPHOSPHOGLYCERATE COMPLEX LOW-SALT STRUCTURE AT 2.-5 ANGSTROM RESOLUTION

被引：61

作者：

RICHARD, V ^{[1
]}

DODSON, GG ^{[1
]}

MAUGUEN, Y ^{[1
]}

机构：

[1] UNIV YORK,DEPT CHEM,YORK YO1 5DD,N YORKSHIRE,ENGLAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 233卷 / 02期

关键词：

HEMOGLOBIN; ALLOSTERIC EFFECTOR; 2,3-DIPHOSPHOGLYCERATE; CRYSTALLOGRAPHY; BINDING SITE;

D O I：

10.1006/jmbi.1993.1505

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The haemoglobin-2,3-diphosphoglycerate complex structure has been solved at 2.5 Å resolution using crystals grown from low-salt solutions. The results show some important differences with the precedent haemoglobin-2,3-diphosphoglycerate high-salt structure solved by Arnone. First, we observe a loss of symmetry in the binding site, secondly both of the lysine residues 82β interact with 2,3-diphosphoglycerate at the same time, each making two contacts. This level of interaction is in agreement with the functional behaviour of natural haemoglobin mutants with mutations at the 2,3-diphosphoglycerate binding site.

引用

页码：270 / 274

页数：5

共 14 条

[1] X-RAY-DIFFRACTION STUDY OF BINDING OF 2,3-DIPHOSPHOGLYCERATE TO HUMAN DEOXYHEMOGLOBIN [J].