REDUCING THE OVERLAP PROBLEM IN THE PROTON NMR-SPECTRA OF OLIGOSACCHARIDES BY APPLICATION OF PSEUDO-4-DIMENSIONAL HOMONUCLEAR HOHAHA-HOHAHA-COSY

被引：9

作者：

RUTHERFORD, TJ

HOMANS, SW

机构：

[1] Department of Biochemistiy, University of Dundee

来源：

GLYCOBIOLOGY | 1992年 / 2卷 / 04期

基金：

英国惠康基金;

关键词：

4-DIMENSIONAL NMR/N-LINKED GLYCAN; SEROTRANSFERRIN;

D O I：

10.1093/glycob/2.4.293

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new homonuclear NMR experiment is described for the assignment of the proton NMR spectra of oligosaccharides, namely HOHAHA-HOHAHA-COSY (where HOHAHA is homonuclear Hartmann-Hahn spectroscopy and COSY is correlated spectroscopy). While this technique may formally be thought of as a four-dimensional NMR experiment, by use of selective pulses it is demonstrated that the analogous pseudo-four-dimensional experiment is a valuable improvement over conventional three-dimensional HOHAHA-COSY, in that the degree of resonance overlap is markedly reduced by the dispersion of resonances into a fourth effective dimension. The technique is demonstrated by application to the biantennary nonasaccharide Ga-beta-1-4-GlcNAc-beta-1-2Man-alpha-1-6(Gal-beta-1-4GlcNAc-beta-1-2Man-alpha-1-3)Man-beta-1-4-GlcNAc-beta-1-4glcNAc.

引用

收藏

页码：293 / 298

页数：6

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