PANCREASTATIN INCREASES CYTOSOLIC CA2+ IN INSULIN SECRETING RINM5F CELLS

被引：24

作者：

SANCHEZMARGALET, V

LUCAS, M

GOBERNA, R

机构：

[1] Department of Medical Biochemistry and Molecular Biology, Medical School of Sevilla, Hospital Virgen Macarena, Sevilla

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1992年 / 88卷 / 1-3期

关键词：

PANCREASTATIN; CALCIUM; ATP; ALPHA-ADRENERGIC RECEPTOR BLOCKER; INSULIN SECRETING CELL LINE (RINM5F);

D O I：

10.1016/0303-7207(92)90017-Z

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have investigated the effect of pancreastatin on cytosolic Ca2+ concentration in the insulin secreting cell line RINm5F. Changes in [Ca2+]i induced by pancreastatin were detected by Fluo-3 fluorescence using both flow cytometry and batch analysis measurements, and turned out to be from 90 to 315 nM equivalent to 80% of that caused by ATP. which increased [Ca2+]i from 90 nM to 400 nM. This effect of pancreastatin did not depend on extracellular calcium and was not mediated by alpha-adrenergic receptors since it was not prevented by the alpha-blocker yohimbine. It is concluded that pancreastatin has a role in the homeostasis of free cytosolic calcium in the insulin secreting cell line Rinm5F.

引用

页码：129 / 133

页数：5

共 21 条

[1] PANCREASTATIN INHIBITS INSULIN-SECRETION AND STIMULATES GLUCAGON-SECRETION IN MICE [J].

AHREN, B ;

LINDSKOG, S ;

TATEMOTO, K ;

EFENDIC, S .

DIABETES, 1988, 37 (03) :281-285

[2] MECHANISM OF GALANIN-INHIBITED INSULIN RELEASE - OCCURRENCE OF A PERTUSSIS-TOXIN-SENSITIVE INHIBITION OF ADENYLATE-CYCLASE [J].

AMIRANOFF, B ;

LORINET, AM ;

LAGNYPOURMIR, I ;

LABURTHE, M .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 177 (01) :147-152

[3] EXTRACELLULAR ATP INCREASES CYTOPLASMIC FREE CA-2+ CONCENTRATION IN CLONAL INSULIN-PRODUCING RINM5F CELLS - A MECHANISM INVOLVING DIRECT INTERACTION WITH BOTH RELEASE AND REFILLING OF THE INOSITOL 1,4,5-TRISPHOSPHATE-SENSITIVE CA-2+ POOL [J].

ARKHAMMAR, P ;

HALLBERG, A ;

KINDMARK, H ;

NILSSON, T ;

RORSMAN, P ;

BERGGREN, PO .

BIOCHEMICAL JOURNAL, 1990, 265 (01) :203-211

[4] ISLET ELECTRICAL PACEMAKER RESPONSE TO ALPHA-ADRENERGIC STIMULATION [J].

COOK, DL ;

PERARA, E .

DIABETES, 1982, 31 (11) :985-990

[5] PANCREASTATIN AND ISLET HORMONE-RELEASE [J].

EFENDIC, S ;

TATEMOTO, K ;

MUTT, V ;

QUAN, C ;

CHANG, D ;

OSTENSON, CG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (20) :7257-7260

[6] PANCREASTATIN, A PRESUMED PRODUCT OF CHROMOGRANIN-A (SECRETORY PROTEIN-I) PROCESSING, INHIBITS SECRETION FROM PORCINE PARATHYROID CELLS IN CULTURE [J].

FASCIOTTO, BH ;

GORR, SU ;

DEFRANCO, DJ ;

LEVINE, MA ;

COHN, DV .

ENDOCRINOLOGY, 1989, 125 (03) :1617-1622

[7] EFFECT OF PANCREASTATIN ON PANCREATIC ENDOCRINE FUNCTION IN THE CONSCIOUS RAT [J].

FUNAKOSHI, A ;

MIYASAKA, K ;

KITANI, K ;

TATEMOTO, K .

REGULATORY PEPTIDES, 1989, 24 (03) :225-231

[8]

IANCANGELO AL, 1988, ENDOCRINOLOGY, V122, P2339

[9] PANCREASTATIN INHIBITS INSULIN RELEASE FROM RIN M 5F CELLS - REVERSION BY PERTUSSIS TOXIN [J].

LORINET, AM ;

TATEMOTO, K ;

LABURTHE, M ;

AMIRANOFF, B .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1989, 160 (03) :405-407

[10] OPPOSITE EFFECT OF CYTOCHALASIN-B ON AGONIST-INDUCED RESPIRATORY BURST IN NEUTROPHILS AND MONOCYTES [J].

LUCAS, M ;

SOLANO, F .

BIOCHEMICAL PHARMACOLOGY, 1991, 42 (02) :425-430

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