GENOMIC MAPPING OF INTRACISTERNAL A-PARTICLE PROVIRAL ELEMENTS

被引：37

作者：

LUEDERS, KK

FRANKEL, WN

MIETZ, JA

KUFF, EL

机构：

[1] NCI,TUMOR VIRUS BIOL LAB,BETHESDA,MD 20892

[2] JACKSON LAB,BAR HARBOR,ME 04609

来源：

MAMMALIAN GENOME | 1993年 / 4卷 / 02期

关键词：

D O I：

10.1007/BF00290429

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Intracisternal A-particle (IAP) proviral elements are moderately reiterated and widely dispersed in the mouse genome. Oligonucleotide probes have been derived from three distinctive IAP element subfamilies (LS elements) that are transcriptionally active in normal mouse B- and T-cells. In HindIII digests, LS element-specific oligonucleotides each react with a limited number of restriction fragments that represent junctions between proviral and flanking DNA. These fragments have characteristic strain distribution patterns (SDPs) which are polymorphic in the DNAs of different mouse strains. We have established chromosomal assignments for 44 LS proviral loci by comparing their SDPs with those of known genetic markers in the BXD set of RI mouse strains. Some of the loci have also been scored in the CXB RI set. The IAP LS loci can provide a significant number of markers with a recognized genetic organization to the mouse genome map.

引用

页码：69 / 77

页数：9

共 46 条

[1] CHROMOSOMAL LOCATIONS OF GENES ENCODING 2',3' CYCLIC-NUCLEOTIDE 3'-PHOSPHODIESTERASE AND GLIAL FIBRILLARY ACIDIC PROTEIN IN THE MOUSE [J].