THE ROLE OF THE PRESERVED SEQUENCES OF DAM METHYLASE

被引：27

作者：

GUYOT, JB

GRASSI, J

HAHN, U

GUSCHLBAUER, W

机构：

[1] CTR ETUD SACLAY, DEPT BIOL CELLULAIRE & MOLEC, SERV BIOCHIM & GENET MOLEC, BAT 142, F-91191 GIF SUR YVETTE, FRANCE

[2] CTR ETUD SACLAY, DEPT RECH IMAGERIE PHARMACOL & PHYSIOL, SERV PHARMACOL & IMMUNOL, F-91191 GIF SUR YVETTE, FRANCE

[3] FREE UNIV BERLIN, INST KRISTALLOG, W-1000 BERLIN 33, GERMANY

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 14期

关键词：

D O I：

10.1093/nar/21.14.3183

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have undertaken a site directed mutational analysis of two of the preserved regions in the amino acid sequence of Dam methylase in order to characterize their role. Mutations in region IV (sequence DPPY) abolish catalytic activity and greatly affect AdoMet crosslinking. Mutants in region III display a lowered specific activity with an unchanged AdoMet crosslinking capacity. We have also made a series of deletions both at the N and C terminal parts of the protein, which have been found to provide inactive enzyme. We discuss the significance of these results for the understanding of the functional properties of the enzyme.

引用

页码：3183 / 3190

页数：8

共 47 条

[1] CONSTRUCTION AND USE OF CHIMERIC SPR PHI-3T DNA METHYLTRANSFERASES IN THE DEFINITION OF SEQUENCE RECOGNIZING ENZYME REGIONS [J].

BALGANESH, TS ;

REINERS, L ;

LAUSTER, R ;

NOYERWEIDNER, M ;

WILKE, K ;

TRAUTNER, TA .

EMBO JOURNAL, 1987, 6 (11) :3543-3549

[2] THE GREAT GATC - DNA METHYLATION IN ESCHERICHIA-COLI [J].

BARRAS, F ;

MARINUS, MG .

TRENDS IN GENETICS, 1989, 5 (05) :139-143

[3] ORGANIZATION OF MULTISPECIFIC DNA METHYLTRANSFERASES ENCODED BY TEMPERATE BACILLUS-SUBTILIS PHAGES [J].

BEHRENS, B ;

NOYERWEIDNER, M ;

PAWLEK, B ;

LAUSTER, R ;

BALGANESH, TS ;

TRAUTNER, TA .

EMBO JOURNAL, 1987, 6 (04) :1137-1142

[4] ALLOSTERIC AND CATALYTIC BINDING OF S-ADENOSYLMETHIONINE TO ESCHERICHIA-COLI DNA ADENINE METHYLTRANSFERASE MONITORED BY H-3 NMR [J].

BERGERAT, A ;

GUSCHLBAUER, W ;

FAZAKERLEY, GV .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (15) :6394-6397

[5]

BERGERAT A, 1989, J BIOL CHEM, V264, P4064

[6]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[7] THE ISOLATION AND CHARACTERIZATION OF THE ESCHERICHIA-COLI DNA ADENINE METHYLASE (DAM) GENE [J].

BROOKS, JE ;

BLUMENTHAL, RM ;

GINGERAS, TR .

NUCLEIC ACIDS RESEARCH, 1983, 11 (03) :837-851

[8]

Chandrasegaran S, 1988, STRUCTURE EXPRESSION, V1, P149

[9] DIRECT IDENTIFICATION OF THE ACTIVE-SITE NUCLEOPHILE IN A DNA (CYTOSINE-5)-METHYLTRANSFERASE [J].

CHEN, L ;

MACMILLAN, AM ;

CHANG, W ;

EZAZNIKPAY, K ;

LANE, WS ;

VERDINE, GL .

BIOCHEMISTRY, 1991, 30 (46) :11018-11025

[10] CONTROLLING BASAL EXPRESSION IN AN INDUCIBLE T7 EXPRESSION SYSTEM BY BLOCKING THE TARGET T7 PROMOTER WITH LAC REPRESSOR [J].

DUBENDORFF, JW ;

STUDIER, FW .

JOURNAL OF MOLECULAR BIOLOGY, 1991, 219 (01) :45-59

← 1 2 3 4 5 →