IMPROVED HISTOCHEMICAL METHOD FOR DEMONSTRATION OF ACTIVITY OF ALPHA-GLUCAN PHOSPHORYLASE .I. USE OF GLUCOSYL ACCEPTOR DEXTRAN

被引：75

作者：

MEIJER, AEF

机构：

[1] Laboratory of Pathological Anatomy, Histochemical and Biochemical Section, University of Amsterdam

来源：

HISTOCHEMIE | 1968年 / 12卷 / 03期

关键词：

D O I：

10.1007/BF00306002

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A modified method for the histochemical demonstration of the activity of α-glucan phosphorylase is described. In the histochemical system the enzyme catalyses the synthesis of glycogen by transglucosylation from α-d-glucosylphosphate. The incubation medium contains dextran as glucosyl acceptor. Therefore, in contrast with the unmodified method, the new technique is able to demonstrate the activity of phosphorylase in ischaemic glycogen-depleted tissues. © 1968 Springer-Verlag.

引用

页码：244 / &

共 17 条

[1]

BAJUSZ E, 1964, ACTA HISTOCHEM, V18, P222

[2]

COLOWICK SP, 1955, METHODS ENZYMOLOG ED, V1, P200

[3] MUSCLE PHOSPHORYLASE [J].