FLUORESCENCE DETECTION OF CARBON-CENTERED RADICALS IN AQUEOUS-SOLUTION

被引：58

作者：

KIEBER, DJ ^{[1
]}

BLOUGH, NV ^{[1
]}

机构：

[1] WOODS HOLE OCEANOG INST,DEPT CHEM,WOODS HOLE,MA 02543

来源：

FREE RADICAL RESEARCH COMMUNICATIONS | 1990年 / 10卷 / 1-2期

关键词：

Aqueous photochemistry; Fluorescence; High performance liquid chromatography; Nitroxides; Radicals;

D O I：

10.3109/10715769009145940

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, highly sensitive method for the simultaneous determination of arrays of carbon-centered radicals in aqueous systems is described. Radicals are efficiently trapped by an amino-nitroxide to form stable products which are then reacted with fluorescamine to produce highly fluorescent adducts. The adducts are easily separated by reversed-phase high performance liquid chromatography. The detection limit for individual radical adducts (0.5 to 2nM) is two to three orders of magnitude lower than those of current methods employing electron paramagnetic resonance detection. Results on the photolysis of ketones and z-keto acids demonstrate the potential of this technique. This approach should be widely applicable to the study of radical processes in biological and chemical systems. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：109 / 117

页数：9

共 27 条

[1] KINETICS OF THE COUPLING REACTIONS OF THE NITROXYL RADICAL 1,1,3,3-TETRAMETHYLISOINDOLINE-2-OXYL WITH CARBON-CENTERED RADICALS [J].