PERSISTENCE OF METHYLATED BASES IN RIBONUCLEIC-ACID OF SYRIAN GOLDEN-HAMSTER LIVER AFTER ADMINISTRATION OF DIMETHYLNITROSAMINE

被引:12
作者
MARGISON, GP [1 ]
MARGISON, JM [1 ]
MONTESANO, R [1 ]
机构
[1] INT AGCY RES CANC,CHEM CARCINOGENESIS UNIT,F-69372 LYON 2,FRANCE
关键词
D O I
10.1042/bj1770967
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Syrian golden hamster liver ribosomal RNA was isolated up to 96 h after administration of [14C]dimethylnitrosamine at 25 mg/kg or 2.5 mg/kg body weight. The chemical alkylation products, 7-methylguanine, 3-methylcytosine, O6-methylguanosine and 1-methyladenosine, were measured after acidic or enzymic hydrolysis of the RNA to bases or mononucleosides followed by ion-exchange chromatography. Between 7 and 96 h, the relative amounts of alkylation products did not change with time even though the absolute amounts fell by approx. 80% and 51% after the high and low doses respectively. The results suggest that base specific excision repair does not exist for RNA alkylation products in this experimental system.
引用
收藏
页码:967 / 973
页数:7
相关论文
共 32 条
[1]  
CAPPS MJ, 1973, BIOCH BIOPHYS ACTA, V186, P229
[2]   REACTION OF CARCINOGEN DIMETHYLNITROSAMINE WITH NUCLEIC ACIDS IN VIVO [J].
CRADDOCK, VM ;
MAGEE, PN .
BIOCHEMICAL JOURNAL, 1963, 89 (01) :32-&
[3]   FATE OF 7[14C]-METHYLGUANINE AFTER ADMINISTRATION TO RAT [J].
CRADDOCK, VM ;
MATTOCKS, AR ;
MAGEE, PN .
BIOCHEMICAL JOURNAL, 1968, 109 (01) :75-&
[4]  
CRAIG AW, 1971, BIOCH J, V123, P34
[5]  
DUTTON AH, 1956, J CHEM SOC, P1892
[6]  
GALBRAITH A, BIOCH BIOPHYS ACTA
[7]  
Hall RH, 1971, MODIFIED NUCLEOSIDES
[8]   METHYLATION OF DEOXYRIBONUCLEIC ACID BY DIAZOMETHANE [J].
KRIEK, E ;
EMMELOT, P .
BIOCHIMICA ET BIOPHYSICA ACTA, 1964, 91 (01) :59-&
[9]   FURTHER STUDIES ON ALKYLATION OF NUCLEIC ACIDS AND THEIR CONSTITUENT NUCLEOTIDES [J].
LAWLEY, PD ;
BROOKES, P .
BIOCHEMICAL JOURNAL, 1963, 89 (01) :127-+
[10]   SOME CHEMICAL ASPECTS OF DOSE-RESPONSE RELATIONSHIPS IN ALKYLATION MUTAGENESIS [J].
LAWLEY, PD .
MUTATION RESEARCH, 1974, 23 (03) :283-295