A BLOTTING METHOD FOR MONITORING THE FORMATION OF CHEMICALLY-INDUCED DNA PROTEIN COMPLEXES

被引：16

作者：

COHEN, MD

MILLER, CA

XU, LS

SNOW, ET

COSTA, M

机构：

[1] Department of Environmental Medicine, New York University Medical Center, New York, NY 10016

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 186卷 / 01期

关键词：

D O I：

10.1016/0003-2697(90)90562-N

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The formation and identification of DNA-protein crosslinks are usually detected by filter binding assays such as alkaline elution. We describe a modified blotting method to selectively identify DNA-protein complexes (DPCs) formed in vitro by either Cr3+ ion or formaldehyde. This protocol allows DPC formation in vitro to be assayed with various chemical agents, requires minimal usage of radioactivity, and is performed in a shorter time frame than that commonly used to resolve DPCs from free proteins and unbound DNA. © 1990.

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页码：1 / 7

页数：7

相关论文

共 20 条

[1] INTRACELLULAR CHROMIUM REDUCTION [J].

ARSLAN, P ;

BELTRAME, M ;

TOMASI, A .

BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 931 (01) :10-15

[2] VANADIUM INHIBITION OF YEAST GLUCOSE-6-PHOSPHATE-DEHYDROGENASE [J].

COHEN, MD ;

SEN, AC ;

WEI, CI .

INORGANICA CHIMICA ACTA-BIOINORGANIC CHEMISTRY, 1987, 138 (03) :179-186

[3]

CONNETT PH, 1983, STRUCT BOND, V54, P93

[4] A RELIABLE METHOD FOR THE RECOVERY OF DNA FRAGMENTS FROM AGAROSE AND ACRYLAMIDE GELS [J].

DRETZEN, G ;

BELLARD, M ;

SASSONECORSI, P ;

CHAMBON, P .

ANALYTICAL BIOCHEMISTRY, 1981, 112 (02) :295-298

[5] DNA-PROTEIN CROSS-LINKING BY CHROMIUM SALTS [J].

FORNACE, AJ ;

SERES, DS ;

LECHNER, JF ;

HARRIS, CC .

CHEMICO-BIOLOGICAL INTERACTIONS, 1981, 36 (03) :345-354

[6] A GEL-ELECTROPHORESIS METHOD FOR QUANTIFYING THE BINDING OF PROTEINS TO SPECIFIC DNA REGIONS - APPLICATION TO COMPONENTS OF THE ESCHERICHIA-COLI LACTOSE OPERON REGULATORY SYSTEM [J].

GARNER, MM ;

REVZIN, A .

NUCLEIC ACIDS RESEARCH, 1981, 9 (13) :3047-3060

[7] INTERACTION OF VANADATE WITH MONOSACCHARIDES AND NUCLEOSIDES - A MULTINUCLEAR NMR-STUDY [J].

GERALDES, CFGC ;

CASTRO, MMCA .

JOURNAL OF INORGANIC BIOCHEMISTRY, 1989, 35 (02) :79-93

[8] STUDIES ON THE MECHANISM OF NICKEL-INDUCED HETEROCHROMATIN DAMAGE - EFFECT ON SPECIFIC DNA-PROTEIN INTERACTIONS [J].

IMBRA, RJ ;

LATTA, DM ;

COSTA, M .

TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY, 1989, 22 (1-4) :167-179

[9]

JENNETTE KW, 1981, ENV HLTH PERSPECT, V40, P223

[10]

Maniatis T., 1982, MOL CLONING LABORATO, P76