BULK MODIFIED ENZYME ELECTRODES FOR REAGENTLESS DETECTION OF PEROXIDES

被引：120

作者：

WOLLENBERGER, U

WANG, J

OZSOZ, M

GONZALEZROMERO, E

SCHELLER, F

机构：

[1] NEW MEXICO STATE UNIV,DEPT CHEM,LAS CRUCES,NM 88003

[2] CENT INST MOLEC BIOL,O-1115 BERLIN,GERMANY

来源：

BIOELECTROCHEMISTRY AND BIOENERGETICS | 1991年 / 26卷 / 02期

关键词：

D O I：

10.1016/0302-4598(91)80030-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Reagentless bulk-type biosensors for peroxides based on the incorporation of horse-radish peroxidase into carbon paste and graphite-epoxy matrices are described. Mediatorless detection of organic peroxides is reported for the first time. The principle of measurement is based on the electrocatalytic current arising in dependence on the peroxides level in micromolar concentration range. The polishable graphite-epoxy biosensors exhibit a life time of more than 40 days. Analogous observations are reported for a fungal peroxidase.

引用

页码：287 / 296

页数：10

共 15 条

[1]

BARMAN TE, 1969, ENZYME HDB, V1, P234

[2] FUNCTION AND MECHANISM OF ACTION OF PEROXIDASES [J].