FLOW-INJECTION AMPEROMETRIC BIOSENSOR BASED ON IMMOBILIZED L-LYSINE-ALPHA-OXIDASE FOR L-LYSINE DETERMINATION

被引：26

作者：

SIMONIAN, AL

BADALIAN, IE

BEREZOV, TT

SMIRNOVA, IP

KHADUEV, SH

机构：

[1] YEREVAN PHYS INST, BIOSENSORS LAB, YEREVAN 375036, ARMENIA

[2] PATRICE LUMUMBA PEOPLES FRIENDSHIP UNIV, MOSCOW, RUSSIA

来源：

ANALYTICAL LETTERS | 1994年 / 27卷 / 15期

关键词：

L-LYSINE; FLOW-INJECTION ANALYSIS; L-LYSINE-ALPHA-OXIDASE; AMPEROMETRIC BIOSENSOR;

D O I：

10.1080/00032719408000296

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Amperometric flow-injection analytical system for quantitation of low L-lysine concentration was described. L-lysine-alpha-oxidase from Trichoderme sp. was immobilized by covalent linking on silica gel. The shift in oxygen concentration during enzymatic reaction was detected with a Clark membrane electrode. The rate of oxygen consumption was linearly dependent on L-lysine concentration over the 0.20 - 5.5 mM. Response time was 14 s, the total assay time about 2 min. The influences of pH, ionic strength and nature of the buffer on enzyme catalytic activity were tested. The analysis conditions were optimized. The immobilized L-lysine-alpha-oxidase retained the catalytic activity for about 5 months.

引用

页码：2849 / 2860

页数：12

共 15 条

[1] BIOSENSORS - WHAT REAL PROGRESS IS BEING MADE [J].