Strategies for manipulating metabolic fluxes in biotechnology

被引:57
作者
CornishBowden, A [1 ]
Hofmeyr, JHS [1 ]
Cardenas, ML [1 ]
机构
[1] UNIV STELLENBOSCH, DEPT BIOCHEM, STELLENBOSCH 7600, SOUTH AFRICA
关键词
D O I
10.1006/bioo.1995.1030
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Strategies for biotechnologically manipulating metabolic fluxes are critically examined in relation to a model system. The common idea of first identifying the rate-limiting enzyme in the biosynthetic pathway to a desired end-product, and then increasing its activity, is shown to be completely ineffective; such manipulation typically produces only trivial changes in flux. Manipulating the activities of all of the enzymes in a biosynthetic pathway by amounts calculated to increase a desired flux while leaving all other fluxes and all concentrations unchanged is potentially effective, and can be applied to any system without regard to its regulatory design. however, it requires accurate knowledge of the initial state of the system and the ability to make precise changes to numerous activities. The classical information about the regulatory mechanisms that exist in living organisms suggests that one can make much simpler manipulations, involving only the steps that remove the desired end-product, with almost equally satisfactory results. (C) 1995 Academic Press, Inc.
引用
收藏
页码:439 / 449
页数:11
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