NITRATE REGULATION OF ANAEROBIC RESPIRATORY GENE-EXPRESSION IN NARX DELETION MUTANTS OF ESCHERICHIA-COLI K-12

Previous studies have shown that narL+ is required for nitrate regulation of anaerobic respiratory enzyme synthesis, including formate dehydrogenase-N, nitrate reductase, and fumarate reductase. Insertions in the closely linked narX gene decrease, but do not abolish, nitrate regulation of anaerobic enzyme synthesis. Analysis of sequence similarities suggests that NarX and NarL comprise a two-component regulatory pair. We constructed lacZ operon and gene fusions to investigate the operon structure of narXL. We found evidence for a complete operon with at least two promoters: P(XL)-narX-P(L)-narL. We also investigated the role of NarX in nitrate regulation of anaerobic respiratory enzyme synthesis by constructing nonpolar loss of function narX alleles. These deletions were studied on narL+ λ specialized transducing bacteriophage. The narX deletions had no effect on nitrate regulation in Δ(narXL) strains. This finding suggests that the subtle effects of previously studied narX insertions are due to decreased expression of narL and that narX+ is not essential for normal nitrate regulation. The role of NarX in nitrate regulation remains to be determined.