METABOLISM OF 22-OXACALCITRIOL BY A VITAMIN-D-INDUCIBLE PATHWAY IN CULTURED PARATHYROID CELLS

被引：23

作者：

BROWN, AJ ^{[1
]}

BERKOBEN, M ^{[1
]}

RITTER, C ^{[1
]}

KUBODERA, N ^{[1
]}

NISHII, Y ^{[1
]}

SLATOPOLSKY, E ^{[1
]}

机构：

[1] CHUGAI PHARMACEUT LTD,TOKYO,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 189卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)92266-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Catabolism of 22-oxacalcitriol (OCT) in parathyroid cells was compared to that of the parent hormone, 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]. Catabolism of both compounds was greatly accelerated by pretreatment of the cells with 1,25-(OH)2D3 or OCT. The rate of degradation of OCT was slightly greater than that of 1,25-(OH)2D3. Excess unlabeled OCT or 1,25-(OH)2D3 inhibited metabolism of both tritiated substrates. Ketoconazole, a cytochrome P450 inhibitor, blocked catabolism of both compounds. The major OCT metabolite appeared to be 1,20-dihydroxy-22,23,24,25,26,27-hexanor-vitamin D3 which was not active in suppressing PTH secretion. We conclude that OCT appears to be metabolized by the same vitamin D-inducible side chain oxidation pathway that catabolizes other vitamin D compounds and that its higher than expected suppression of PTH secretion is not due to slower cellular metabolism. © 1992.

引用

页码：759 / 764

页数：6

共 21 条

[1] A SYNTHETIC ANALOG OF VITAMIN-D3, 22-OXA-1-ALPHA,25-DIHYDROXYVITAMIN-D3, IS A POTENT MODULATOR OF INVIVO IMMUNOREGULATING ACTIVITY WITHOUT INDUCING HYPERCALCEMIA IN MICE [J].