POTENTIATION OF NITRIC-OXIDE FORMATION FOLLOWING BILATERAL CAROTID OCCLUSION AND FOCAL CEREBRAL-ISCHEMIA IN THE RAT - INVIVO DETECTION OF THE NITRIC-OXIDE RADICAL BY ELECTRON-PARAMAGNETIC-RESONANCE SPIN-TRAPPING

被引：104

作者：

TOMINAGA, T ^{[1
]}

SATO, S ^{[1
]}

OHNISHI, T ^{[1
]}

OHNISHI, ST ^{[1
]}

机构：

[1] UNIV PENN,SCH MED,DEPT BIOCHEM & BIOPHYS,PHILADELPHIA,PA 19104

来源：

BRAIN RESEARCH | 1993年 / 614卷 / 1-2期

关键词：

NITRIC OXIDE; INVIVO SPIN-TRAPPING; CRYOGENIC ELECTRON PARAMAGNETIC RESONANCE; FOCAL ISCHEMIA; ENDOTHELIUM-DERIVED RELAXING FACTOR; NITRIC OXIDE SYNTHASE INHIBITOR;

D O I：

10.1016/0006-8993(93)91053-U

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We have directly demonstrated in vivo that nitric oxide (NO) is produced in the ischemic rat brain. Using diethyldithiocarbamate and Fe as spin-trapping agents, NO spin adducts were detected by cryogenic electron paramagnetic resonance. The cerebral cortex which was exposed to focal ischemia or bilateral carotid artery occlusion generated an increased amount of spin-adducts of NO radicals (g = 2.039, a hyperfine coupling constant a(N) = 13 gauss). This signal disappeared by the preischemic administration of N(G)-nitro-L-arginine methylester, a NO synthase inhibitor.

引用

页码：342 / 346

页数：5

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