PLASMODIUM-BERGHEI - RECOMBINANT INTERFERON-GAMMA AND THE DEVELOPMENT OF PARASITEMIA AND CEREBRAL-LESIONS IN MALARIA-INFECTED MICE

被引:30
作者
CURFS, JHAJ
VANDERMEIDE, PH
BILLIAU, A
THMEUWISSEN, JHE
ELING, WMC
机构
[1] CATHOLIC UNIV NIJMEGEN,DEPT MED MICROBIOL,POB 9101,6500 HB NIJMEGEN,NETHERLANDS
[2] TNO,INST APPL RADIOBIOL & IMMUNOL,RIJSWIJK,NETHERLANDS
[3] REGA INST,LOUVAIN,BELGIUM
关键词
D O I
10.1006/expr.1993.1078
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Mice infected with Plasmodium berghei K173-parasitized erythrocytes develop severe hypothermia followed by death as a consequence of murine cerebral malaria early in the second week after infection. A single intraperitoneal injection of 105 Units of IFN-γ given between Day 4 and Day 6 postinfection results in a transient decrease of body temperature. No effect on parasitemia and cerebral malaria is obtained by this treatment. Daily injections of relatively low doses of IFN-γ delays the patency of the infection for 2 days. Furthermore the proliferation rate of the parasites is reduced and the development of cerebral malaria is also delayed for 2 days. The reduction of body temperature, as found in untreated infected mice, is absent. Administration of IFN-γ by means of a continuous delivery from intraperitoneally inserted osmotic pumps (1.2 x 104 Units of IFN-γ/24 hr) also delays patency and inhibits parasitemia. Body temperature decreases during infection but mice are protected against the development of cerebral malaria. In nude mice, this treatment inhibits parasitemia to the same extent. However, reduction of body temperature was also prevented. High doses of IFN-γ delivered by osmotic pumps (2.5 x 104 or 105 Units of IFN-γ/24 hr) appear to be lethally toxic in conventional as well as in nude mice, independently of infection. Cerebral malaria-like symptoms are found in these mice. Treatment of infected C57BL/6J mice with antibody to IFN-γ 4 days before and after infection as well as on the day of infection enhances parasitemia but does not affect the development of murine cerebral malaria. Single injections of anti-IFN-γ-antibody 6 hr prior to infection or 7 days after infection have no effect. In CBA/Ca mice, treatment with anti-IFN-γ-antibody enhances parasitemia; furthermore protection against cerebral malaria was obtained in part of the mice.© 1993 Academic press Inc. © 1993 Academic press, Inc.
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页码:212 / 223
页数:12
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