QUANTITATIVE-EVALUATION OF HEPATITIS-C VIRUS-RNA IN PATIENTS WITH CONCURRENT HUMAN-IMMUNODEFICIENCY-VIRUS INFECTIONS

被引：188

作者：

SHERMAN, KE ^{[1
]}

OBRIEN, J ^{[1
]}

GUTIERREZ, AG ^{[1
]}

HARRISON, S ^{[1
]}

URDEA, M ^{[1
]}

NEUWALD, P ^{[1
]}

WILBER, J ^{[1
]}

机构：

[1] CHIRON CORP,EMERYVILLE,CA 94608

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1993年 / 31卷 / 10期

关键词：

D O I：

10.1128/JCM.31.10.2679-2682.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Quantitation of the hepatitis C virus (HCV) provides a powerful epidemiologic and therapeutic method for the evaluation of infected patients. In this study semiquantitative reverse transcriptase polymerase chain reaction (PCR) is compared with a new branched DNA signal amplification methodology. Samples from HCV-infected patients as well as from human immunodeficiency virus-infected patients were evaluated. Reverse transcriptase PCR correlated well with the branched DNA assay (r = 0.7036, P < 0.05). HCV RNA was found to occur at significantly higher titers (P < 0.05) in patients coinfected with the human immunodeficiency virus compared with titers in those infected with HCV alone. Immune status as defined by the CD4+ count was not associated with the observed difference in viral titer.

引用

页码：2679 / 2682

页数：4

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