FUNCTIONAL AND STRUCTURAL INTERACTIONS BETWEEN OSTEOBLASTIC AND PREOSTEOCLASTIC CELLS IN-VITRO

被引：16

作者：

ORLANDINI, SZ

FORMIGLI, L

BENVENUTI, S

LASAGNI, L

FRANCHI, A

MASI, L

BERNABEI, PA

SANTINI, V

BRANDI, ML

机构：

[1] UNIV FLORENCE, SCH MED, DEPT CLIN PHYSIOPATHOL, I-50139 FLORENCE, ITALY

[2] UNIV FLORENCE, SCH MED, DEPT HUMAN ANAT & HISTOL, I-50139 FLORENCE, ITALY

[3] UNIV FLORENCE, SCH MED, INST PATHOL ANAT, I-50139 FLORENCE, ITALY

[4] USL 10D, UO HEMATOL, FLORENCE, ITALY

来源：

CELL AND TISSUE RESEARCH | 1995年 / 281卷 / 01期

关键词：

OSTEOBLAST; PREOSTEOCLASTS; CELL DIFFERENTIATION; HUMAN;

D O I：

10.1007/BF00307956

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Osteoblasts are involved in the bone resorption process by regulating osteoclast maturation and activity. In order to elucidate the mechanisms underlying osteoblast/preosteoclast cell interactions, we developed an in vitro model of co-cultured human clonal cell lines of osteoclast precursors (FLG 29.1) and osteoblastic cells (Saos-2), and evaluated the migratory, adhesive, cytochemical, morphological, and biochemical properties of the co-cultured cells. In Boyden chemotactic chambers, FLG 29.1 cells exhibited a marked migratory response toward the Saos-2 cells. Moreover, they preferentially adhered to the osteoblastic monolayer. Direct co-culture of the two cell types induced: (1) positive staining for tartrate-resistant acid phosphatase in FLG 29.1 cells, (2) a decrease of the alkaline phosphatase activity expressed by Saos-2 cells; (3) the appearance of typical ultrastructural features of mature osteoclasts in FLG 29.1 cells; (4) the release into the culture medium of granulocyte-macrophage colony stimulating factor. The addition of parathyroid hormone to the co-culture further potentiated the differentiation of the preosteoclasts, the cells tending td fuse into large multinucleated elements. These in vitro interactions between osteoblasts and osteoclast precursors offer a new model for studying the mechanisms that control osteoclastogenesis in bone tissue.

引用

页码：33 / 42

页数：10

共 53 条

[1]

Akatsu T., Takahashi N., Udagawa N., Sato K., Nagata N., Moseley J.M., Martin T.J., Suda T., Parathyroid hormone (PTH)-related protein is a potent stimulator of osteoclast-like multinucleated cell formation to the same extend as PTH in mouse marrow cultures, Endocrinology, 125, pp. 20-27, (1989)

[2]

Baron R., Neff L., Van Tran P., Nefussi J.R., Vignery A., Kinetic and cytochemical identification of osteoclast precursors and their differentiation into multinucleated osteoclasts, Am J Pathol, 122, pp. 363-378, (1986)

[3]

Bleiberg I., Glowacki J., Anklesaria P., Greenberger J.S., Origin of stromal cells associated with osteoclast recruitment in s.c. implants of bone particles in chimeric mice, Exp Hematol, 20, pp. 957-961, (1992)

[4]

Boyden S., The chemotactic effect of a mixture of antibody and antigen on polymorphonuclear leukocytes, J Exp Med, 115, pp. 453-466, (1962)

[5]

Braidman I.P., Rothwell C., Webber D.M., Crowe P., Anderson D.C., Location of osteoclast precursors in fetal rat calvaria cultured on collagen gel, J Bone Miner Res, 5, pp. 287-298, (1990)

[6]

Burger E.H., Van Der Meer J.W.M., Nijweide P.J., Osteoclast formation from mononuclear phagocytes: role of bone-forming cells, J Cell Biol, 99, pp. 1901-1906, (1984)

[7]

Canalis E., Effect of glucocorticoids on type I collagen synthesis, alkaline phosphatase activity, and deoxyribonucleic acid content in cultured rat calvariae, Endocrinology, 112, pp. 931-939, (1983)

[8]

Chambers T.J., Fuller K., Bone cells predispose bone surfaces to resorption by exposure of mineral to osteoclastic contact, J Cell Sci, 76, pp. 155-165, (1985)

[9]

Clark S.C., Kamen R., The human hematopoietic colonystimulating factors, Science, 236, pp. 1229-1237, (1987)

[10]

Coccia P.F., Krivit W., Cervenka J., Clawson C., Kersey J.K., Kim T.H., Nesbit M.E., Raay N.K.C., Warkentin P.I., Teitelbaum S.L., Kahn A.J., Brown D.M., Successful bone-marrow transplantation for infantile malignant osteopetrosis, N Engl J Med, 302, pp. 701-708, (1980)

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